SMUF2 rabbit pAb
- Catalog No.:YT7176
- Applications:WB;IHC
- Reactivity:Human;Mouse
- Target:
- SMUF2
- Fields:
- >>Ubiquitin mediated proteolysis;>>Endocytosis;>>Hedgehog signaling pathway;>>TGF-beta signaling pathway
- Gene Name:
- SMURF2
- Protein Name:
- SMUF2
- Human Gene Id:
- 64750
- Human Swiss Prot No:
- Q9HAU4
- Mouse Gene Id:
- 66313
- Mouse Swiss Prot No:
- A2A5Z6
- Immunogen:
- Synthesized peptide derived from human SMUF2 AA range: 505-555
- Specificity:
- This antibody detects endogenous levels of SMUF2 at Human/Mouse
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500-2000;IHC 1:50-300
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Molecular Weight(Da):
- 82kD
- Function:
- domain:The C2 domain is involved in autoinhibition of the catalytic activity by interacting with the HECT domain.,domain:The second and third WW domains are responsible for interaction with the PY-motif of R-SMAD (SMAD1, SMAD2 and SMAD3).,function:E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces
- Subcellular Location:
- Nucleus . Cytoplasm . Cell membrane . Membrane raft . Cytoplasmic in the presence of SMAD7. Colocalizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts.
- Expression:
- Widely expressed.
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- Antibody-FAQs
- Products Images
- Western blot analysis of lysates from MCF-7 cells, primary antibody was diluted at 1:1000, 4°over night
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).