β-actin Monoclonal Antibody(5B7)

    • Catalog No.:YM3028
    • Applications:IF;WB;IHC
    • Reactivity:Human;Rat;Mouse;Mk;Dg;Ch;Hamster;Rabbit;Insect;Pig;Lenok
      • Target:
      • Actin β
      • Fields:
      • >>Rap1 signaling pathway;>>Phagosome;>>Apoptosis;>>Hippo signaling pathway;>>Focal adhesion;>>Adherens junction;>>Tight junction;>>Platelet activation;>>Neutrophil extracellular trap formation;>>Leukocyte transendothelial migration;>>Thermogenesis;>>Regulation of actin cytoskeleton;>>Thyroid hormone signaling pathway;>>Oxytocin signaling pathway;>>Gastric acid secretion;>>Amyotrophic lateral sclerosis;>>Bacterial invasion of epithelial cells;>>Vibrio cholerae infection;>>Pathogenic Escherichia coli infection;>>Shigellosis;>>Salmonella infection;>>Yersinia infection;>>Influenza A;>>Proteoglycans in cancer;>>Hepatocellular carcinoma;>>Hypertrophic cardiomyopathy;>>Arrhythmogenic right ventricular cardiomyopathy;>>Dilated cardiomyopathy;>>Viral myocarditis;>>Fluid shear stress and atherosclerosis
      • Gene Name:
      • ACTB
      • Protein Name:
      • Actin cytoplasmic 1
      • Human Gene Id:
      • 60
      • Human Swiss Prot No:
      • P60709
      • Mouse Swiss Prot No:
      • P60710
      • Immunogen:
      • Synthetic Peptide of β-actin
      • Specificity:
      • The antibody detects endogenous β-actin protein.
      • Formulation:
      • PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
      • Source:
      • Monoclonal, Mouse
      • Dilution:
      • IF 1:50-200 WB 1:5000-20000 IHC 1:200-300
      • Purification:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • ACTB; Actin; cytoplasmic 1; Beta-actin; Actin β;β-Actin;Beta actin
      • Observed Band(KD):
      • 43kD
      • Background:
      • This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008],
      • Function:
      • disease:Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.,function:Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.,miscellaneous:In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.,similarity:Belongs to the
      • Subcellular Location:
      • Cytoplasm, cytoskeleton . Nucleus . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. .
      • Expression:
      • B-cell lymphoma,Brain,Cajal-Retzius cell,Eye,Fetal brain cortex,Foreskin,Hepatocellular car

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      Medium-, long-and medium-chain-type structured lipids ameliorate high-fat diet-induced atherosclerosis by regulating inflammation, adipogenesis, and gut microbiota in ApoE−/− mice." Food & function 11.6 (2020): 5142-5155.

      Zhang, Qianqian, et al. "PPARγ Agonist Pioglitazone Inhibits PDGF-induced Pulmonary Artery Smooth Muscle Cells Proliferation and Migration via Modulating TERT." (2021).

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      Wang, Juan, et al. "Aged Zinc Oxide Nanoparticles Did Not Induce Cytotoxicity Through Apoptosis Signaling Pathway as Fresh NPs." (2020).

      李思汉, et al. "健脾清化舒络中药对慢性萎缩性胃炎大鼠 Sonic Hedgehog 信号通路的影响." 北京中医药大学学报 44.2 (2021): 143-151.

      司晓丽, et al. "芍药苷对 DSS 诱导大鼠慢性溃疡性结肠炎 IL-17 表达的影响." 中国免疫学杂志 37.08: 946.

      Experimental and therapeutic medicine 17.3 (2019): 2349-2358.

      7-Difluoromethoxy-5, 4′-dimethoxy-genistein attenuates macrophages apoptosis to promote plaque stability via TIPE2/TLR4 axis in high fat diet-fed ApoE−/− mice." International Immunopharmacology 96 (2021): 107477.

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      Aberrant methylation‐induced dysfunction of p16 is associated with osteoblast activation caused by fluoride. ENVIRONMENTAL TOXICOLOGY Environ Toxicol. 2019 Jan;34(1):37-47 WB Human osteoblasts

      Neuroprotective effect of Notch pathway inhibitor DAPT against focal cerebral ischemia/reperfusion 3 hours before model establishment. Neural Regeneration Research Neural Regen Res. 2019 Mar; 14(3): 452–461 WB Mouse 1:4000 Brain tissue

      The permeability characteristics and interaction of the main components from Zhizi Bopi decoction in the MDCK cell model. XENOBIOTICA Xenobiotica. 2016;46(8):733-742 WB Dog Madin-Darby canine kidney (MDCK) cell

      The elevated transcription of ADAM19 by the oncohistone H2BE76K contributes to oncogenic properties in breast cancer. JOURNAL OF BIOLOGICAL CHEMISTRY 2021 Jan;296: WB Human 293T cell,MDA-MB-231 cell

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      • Wang, Yue, et al. "Fetal exposure to dichloroacetic acid and impaired cognitive function in the adulthood." Brain and Behavior 10.10 (2020): e01801.
      • Kang, Tze Zhen Evangeline, et al. "The elevated transcription of ADAM19 by the oncohistone H2BE76K contributes to oncogenic properties in breast cancer." Journal of Biological Chemistry 296 (2021).
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      • Tang, Qiusha, et al. "Combination of PEI-Mn0. 5Zn0. 5Fe2O4 nanoparticles and pHsp 70-HSV-TK/GCV with magnet-induced heating for treatment of hepatoma." International journal of nanomedicine 10 (2015): 7129.
      • Wang, Wu M., et al. "MicroRNA-182 regulates neurite outgrowth involving the PTEN/AKT pathway." Frontiers in cellular neuroscience 11 (2017): 96.
      • Liu, Chang, et al. "Mesenchymal Stem Cells Promote the Osteogenesis in Collagen-Induced Arthritic Mice through the Inhibition of TNF-α." Stem cells international 2018 (2018).
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      • Wang, Jun-Jie, et al. "Neuroprotective effect of Notch pathway inhibitor DAPT against focal cerebral ischemia/reperfusion 3 hours before model establishment." Neural regeneration research 14.3 (2019): 452.
      • Wen, Jiexia, et al. "Essential role of Na+/Ca2+ exchanger 1 in smoking-induced growth and migration of esophageal squamous cell carcinoma." Oncotarget 7.39 (2016): 63816.
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      • Immunofluorescence analysis of Hela cell. 1,Cyclin A Polyclonal Antibody(red) was diluted at 1:200(4° overnight). β-actin Monoclonal Antibody(5B7)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
      • Western blot analysis of 1 HEK293 2 SW480 3 HEPG2 4 MCF-7 5 mouse brain 6 Rat brain 7 Hela 8 A549 lysates, primary antibody was diluted at 1:5000, 4° over night, secondary antibody(cat: RS23910 was diluted at 1:10000, 37° 1hour.
      • Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,β-actin Monoclonal Antibody(5B7) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,β-actin Monoclonal Antibody(5B7) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,β-actin Monoclonal Antibody(5B7) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Western blot analysis of Hela (1) Rat brain (2) Mouse brain (3) Chicken lung (4) and Rabbit testis (5), diluted at 1:10000.
      • IHC Staining of Human ovary tissue, diluted at 1:200