β-actin Monoclonal Antibody(5B7)

    • Catalog No.:YM3028
    • Applications:IF/ICC;WB;IHC-p
    • Reactivity:Human;Rat;Mouse;Mk;Dg;Ch;Hamster;Rabbit;Insect
      • Fields:
      • >>Rap1 signaling pathway;>>Phagosome;>>Apoptosis;>>Hippo signaling pathway;>>Focal adhesion;>>Adherens junction;>>Tight junction;>>Platelet activation;>>Neutrophil extracellular trap formation;>>Leukocyte transendothelial migration;>>Thermogenesis;>>Regulation of actin cytoskeleton;>>Thyroid hormone signaling pathway;>>Oxytocin signaling pathway;>>Gastric acid secretion;>>Amyotrophic lateral sclerosis;>>Bacterial invasion of epithelial cells;>>Vibrio cholerae infection;>>Pathogenic Escherichia coli infection;>>Shigellosis;>>Salmonella infection;>>Yersinia infection;>>Influenza A;>>Proteoglycans in cancer;>>Hepatocellular carcinoma;>>Hypertrophic cardiomyopathy;>>Arrhythmogenic right ventricular cardiomyopathy;>>Dilated cardiomyopathy;>>Viral myocarditis;>>Fluid shear stress and atherosclerosis
      • Gene Name:
      • ACTB
      • Protein Name:
      • Actin cytoplasmic 1
      • Human Gene Id:
      • 60
      • Human Swiss Prot No:
      • P60709
      • Mouse Swiss Prot No:
      • P60710
      • Immunogen:
      • Synthetic Peptide of β-actin
      • Specificity:
      • The antibody detects endogenous β-actin protein.
      • Formulation:
      • PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
      • Source:
      • Monoclonal, Mouse
      • Dilution:
      • IF: 1:50-200 WB: 1:5000-20000 IHC: 1:200-300
      • Purification:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • ACTB; Actin; cytoplasmic 1; Beta-actin; Actin β;β-Actin;Beta actin
      • Observed Band(KD):
      • 43
      • Background:
      • This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008],
      • Function:
      • disease:Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.,function:Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.,miscellaneous:In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.,similarity:Belongs to the
      • Subcellular Location:
      • Cytoplasm, cytoskeleton . Nucleus . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. .
      • Expression:
      • B-cell lymphoma,Brain,Cajal-Retzius cell,Eye,Fetal brain cortex,Foreskin,Hepatocellular car

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      The elevated transcription of ADAM19 by the oncohistone H2BE76K contributes to oncogenic properties in breast cancer. JOURNAL OF BIOLOGICAL CHEMISTRY 2021 Jan;296: WB Human 293T cell,MDA-MB-231 cell

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      7-Difluoromethoxy-5,4′-dimethoxy-genistein attenuates macrophages apoptosis to promote plaque stability via TIPE2/TLR4 axis in high fat diet-fed ApoE−/− mice. INTERNATIONAL IMMUNOPHARMACOLOGY Int Immunopharmacol. 2021 Jul;96:107477 WB Mouse 1 : 5000 RAW264.7 cell

      Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 Is an Important Surface Attachment Factor That Facilitates Entry of Middle East Respiratory Syndrome Coronavirus. JOURNAL OF VIROLOGY J Virol. 2016 Oct;: WB Human A549 cell

      Amelioration of Ambient Particulate Matter (PM2.5)-Induced Lung Injury in Rats by Aerobic Exercise Training. Frontiers in Physiology Front Physiol. 2021; 12: 731594 WB Rat 1:500 Lung

      MicroRNA-182 Regulates Neurite Outgrowth Involving the PTEN/AKT Pathway. Frontiers in Cellular Neuroscience 2017 Apr 10 WB Mouse 1:2000 neurons

      Mechanism by which Huoxue Tongluo Qiwei Decoction improves the erectile function of rats with diabetic erectile dysfunction. JOURNAL OF ETHNOPHARMACOLOGY J Ethnopharmacol. 2022 Jan;283:114674 WB Rat Cavernosum tissue

      Trigeminal neuropathy causes hypomyelination in the anterior cingulate cortex, disrupts the synchrony of neural circuitry, and impairs decision-making in male rats. JOURNAL OF NEUROSCIENCE RESEARCH J Neurosci Res. 2021 Oct;99(10):2721-2742 WB Rat 1:5000 Brain

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      Irisin ameliorates nicotine-mediated atherosclerosis via inhibition of the PI3K pathway. Annals of Translational Medicine Ann Transl Med. 2021 May; 9(9): 805 WB Mouse 1:5000 Aortic

      Unraveling proteome changes of Holstein beef M. semitendinosus and its relationship to meat discoloration during post-mortem storage analyzed by label-free mass spectrometry. Journal of Proteomics 2016 Dec 28 WB Bovine 1:5000 Muscle

      High glucose inhibits myogenesis and induces insulin resistance by down-regulating AKT signaling. BIOMEDICINE & PHARMACOTHERAPY 2019 Oct 18 WB Mouse C2C12 cell

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      Effect of leech-centipede medicine on improving erectile function in DIED rats via PKC signalling pathway-related molecules. JOURNAL OF ETHNOPHARMACOLOGY J Ethnopharmacol. 2021 Mar;267:113463 WB Rat Cavernous tissues

      Shuangshen granules attenuate lung metastasis by modulating bone marrow differentiation through mTOR signalling inhibition. JOURNAL OF ETHNOPHARMACOLOGY 2020 Sep 03 WB Mouse 1 : 5000 Lung ,bone marrow tissue Myeloid-derived suppressor cells (MDSCs)

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      miR-187-5p/apaf-1 axis was involved in oxidative stress-mediated apoptosis caused by ammonia via mitochondrial pathway in chicken livers. TOXICOLOGY AND APPLIED PHARMACOLOGY Toxicol Appl Pharm. 2020 Feb;388:114869 WB Chicken 1:1000 liver

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      Activation of AMPK inhibits Galectin-3-induced pulmonary artery smooth muscle cells proliferation by upregulating hippo signaling effector YAP. MOLECULAR AND CELLULAR BIOCHEMISTRY Mol Cell Biochem. 2021 Aug;476(8):3037-3049 WB Rat 1:1000 Pulmonary arterial smooth muscle cells (PASMCs)

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      Tang, Qiusha, et al. "Combination of PEI-Mn0. 5Zn0. 5Fe2O4 nanoparticles and pHsp 70-HSV-TK/GCV with magnet-induced heating for treatment of hepatoma." International journal of nanomedicine 10 (2015): 7129.

      Zhang, Lai-Bo, et al. "Calcitonin protects chondrocytes from lipopolysaccharide-induced apoptosis and inflammatory response through MAPK/Wnt/NF-κB pathways." Molecular immunology 87 (2017): 249-257.

      Ma, Kun, et al. "Neurotoxicity effects of atrazine-induced SH-SY5Y human dopaminergic neuroblastoma cells via microglial activation." Molecular BioSystems 11.11 (2015): 2915-2924.

      艾丽雅, et al. "蒙医不同针刺法对抑郁大鼠前额叶单胺类神经递质及 PKA/CREB/BDNF 通路的影响." 中国医药导报 16.8: 9-12.

      Qianqian, Liu, Ding Shengkai, and Shang Yazhen. "Effect of Scutellaria Baicalensis Georgi Stems and Leaves Flavonoids on Multisite Phosphorylation of tau Protein Induced by Composited Aβ in Rats."

      Medium-, long-and medium-chain-type structured lipids ameliorate high-fat diet-induced atherosclerosis by regulating inflammation, adipogenesis, and gut microbiota in ApoE−/− mice." Food & function 11.6 (2020): 5142-5155.

      Zhang, Qianqian, et al. "PPARγ Agonist Pioglitazone Inhibits PDGF-induced Pulmonary Artery Smooth Muscle Cells Proliferation and Migration via Modulating TERT." (2021).

      Yan, Juntang, et al. "Xiaoqinglong decoction reduces dendritic cell differentiation and regulates the Th1/Th2 balance in a mouse model of allergic asthma." Journal of Traditional Chinese Medical Sciences 7.2 (2020): 133-140.

      Wang, Juan, et al. "Aged Zinc Oxide Nanoparticles Did Not Induce Cytotoxicity Through Apoptosis Signaling Pathway as Fresh NPs." (2020).

      李思汉, et al. "健脾清化舒络中药对慢性萎缩性胃炎大鼠 Sonic Hedgehog 信号通路的影响." 北京中医药大学学报 44.2 (2021): 143-151.

      司晓丽, et al. "芍药苷对 DSS 诱导大鼠慢性溃疡性结肠炎 IL-17 表达的影响." 中国免疫学杂志 37.08: 946.

      Experimental and therapeutic medicine 17.3 (2019): 2349-2358.

      7-Difluoromethoxy-5, 4′-dimethoxy-genistein attenuates macrophages apoptosis to promote plaque stability via TIPE2/TLR4 axis in high fat diet-fed ApoE−/− mice." International Immunopharmacology 96 (2021): 107477.

      Glutathione S‑transferase isozyme alpha 1 is predominantly involved in the cisplatin resistance of common types of solid cancer. ONCOLOGY REPORTS Oncol Rep. 2019 Feb;41(2):989-998 WB Human 1:1000 A549/DPP cell, SKOV3/DDP cell,SGC7901/DDP cell

      Facilitating colorectal cancer cell metastasis by targeted binding of long non-coding RNA ENSG00000231881 with miR-133b via VEGFC signaling pathway. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2018 Dec 20 WB Human 1:1000 HCT-116 cell, Lovo cell

      Aberrant methylation‐induced dysfunction of p16 is associated with osteoblast activation caused by fluoride. ENVIRONMENTAL TOXICOLOGY Environ Toxicol. 2019 Jan;34(1):37-47 WB Human osteoblasts

      Neuroprotective effect of Notch pathway inhibitor DAPT against focal cerebral ischemia/reperfusion 3 hours before model establishment. Neural Regeneration Research Neural Regen Res. 2019 Mar; 14(3): 452–461 WB Mouse 1:4000 Brain tissue

      The permeability characteristics and interaction of the main components from Zhizi Bopi decoction in the MDCK cell model. XENOBIOTICA Xenobiotica. 2016;46(8):733-742 WB Dog Madin-Darby canine kidney (MDCK) cell

      The elevated transcription of ADAM19 by the oncohistone H2BE76K contributes to oncogenic properties in breast cancer. JOURNAL OF BIOLOGICAL CHEMISTRY 2021 Jan;296: WB Human 293T cell,MDA-MB-231 cell

      Taxifolin inhibits keratinocyte proliferation and ameliorates imiquimod-induced psoriasis-like mouse model via regulating cytoplasmic phospholipase A2 and PPAR-γ pathway. INTERNATIONAL IMMUNOPHARMACOLOGY Int Immunopharmacol. 2021 Oct;99:107900 WB Mouse 1:1000 Skin

      Hepatitis B Virus X Protein (HBx) Suppresses Transcription Factor EB (TFEB) Resulting in Stabilization of Integrin Beta 1 (ITGB1) in Hepatocellular Carcinoma Cells. Cancers Cancers. 2021 Jan;13(5):1181 WB Human HepG2 cell,SK-Hep-1 cell,HEK-293FT cell

      Bawei Chenxiang Wan Ameliorates Cardiac Hypertrophy by Activating AMPK/PPAR-α Signaling Pathway Improving Energy Metabolism. Frontiers in Pharmacology Front Pharmacol. 2021 Jun;0:1314 WB Rat Myocardial tissue

      miR-301b-5p and its target gene nfatc2ip regulate inflammatory responses in the liver of rainbow trout (Oncorhynchus mykiss) under high temperature stress ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Gefeng Xu WB Fish

      Effect of the FA2H Gene on cashmere fineness of Jiangnan cashmere goats based on transcriptome sequencing BMC GENOMICS Kechuan Tian WB Goat

      AURKA is a prognostic potential therapeutic target in skin cutaneous melanoma modulating the tumor microenvironment, apoptosis, and hypoxia JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY Xuan Fen Zhang WB Human

      Low LINC02147 expression promotes the malignant progression of oral submucous fibrosis BMC Oral Health Wenjie Li WB Human

      Network Pharmacology, Molecular Docking, and Experimental Validation to Unveil the Molecular Targets and Mechanisms of Compound Fuling Granule to Treat Ovarian Cancer Oxidative Medicine and Cellular Longevity Fangfang Tao WB Human

      Effects of Environmentally Relevant Concentrations of Antipsychotic Drugs (Sulpiride and Clozapine) on Serotonergic and Dopaminergic Neurotransmitter Systems in Octopus Brain Tissue Water Bo Zhang WB Octopus

      Deoxynivalenol damages the intestinal barrier and biota of the broiler chickens BMC Veterinary Research Fan Ruiwen WB Chicken

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      • Immunofluorescence analysis of Hela cell. 1,Cyclin A Polyclonal Antibody(red) was diluted at 1:200(4° overnight). β-actin Monoclonal Antibody(5B7)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
      • Western blot analysis of 1 HEK293 2 SW480 3 HEPG2 4 MCF-7 5 mouse brain 6 Rat brain 7 Hela 8 A549 lysates, primary antibody was diluted at 1:5000, 4° over night, secondary antibody(cat: RS23910 was diluted at 1:10000, 37° 1hour.
      • Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,β-actin Monoclonal Antibody(5B7) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,β-actin Monoclonal Antibody(5B7) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,β-actin Monoclonal Antibody(5B7) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Western blot analysis of Hela (1) Rat brain (2) Mouse brain (3) Chicken lung (4) and Rabbit testis (5), diluted at 1:10000.
      • IHC Staining of Human ovary tissue, diluted at 1:200