β-actin Monoclonal Antibody(5B7)

    • Catalog No.:YM3028
    • Applications:IF/ICC,WB,IHC-p
    • Reactivity:Human,Rat,Mouse,Mk,Dg,Ch,Hamster,Rb,Insect
      • Gene Name:
      • ACTB
      • Protein Name:
      • Actin cytoplasmic 1
      • Human Gene Id:
      • 60
      • Human Swiss Prot No:
      • P60709
      • Mouse Swiss Prot No:
      • P60710
      • Immunogen:
      • Synthetic Peptide of β-actin
      • Specificity:
      • The antibody detects endogenous β-actin protein.
      • Formulation:
      • PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
      • Source:
      • Mouse
      • Dilution:
      • IF: 1:50-200 WB: 1:5000-20000 IHC: 1:200-300
      • Purification:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • ACTB; Actin; cytoplasmic 1; Beta-actin; Actin β;β-Actin;Beta actin
      • MolecularWeight(Da):
      • 41737
      • Observed Band(KD):
      • 43
      • Background:
      • actin beta(ACTB) Homo sapiens This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008],
      • Function:
      • disease:Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.,function:Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.,miscellaneous:In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.,similarity:Belongs to the
      • Subcellular Location:
      • nuclear chromatin,extracellular space,nucleoplasm,cytoplasm,cytosol,cytoskeleton,plasma membrane,focal adhesion,membrane,intracellular ribonucleoprotein complex,cortical cytoskeleton,NuA4 histone acetyltransferase complex,cyt
      • Expression:
      • B-cell lymphoma,Brain,Cajal-Retzius cell,Eye,Fetal brain cortex,Foreskin,Hepatocellular car

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      Dong, Na, et al. "Ethyl pyruvate protects against Salmonella intestinal infection in mice through down-regulation of pro-inflammatory factors and inhibition of TLR4/MAPK pathway." International immunopharmacology 71 (2019): 155-163.

      Song, Zonggong, et al. "miR-352 participates in the regulation of trypsinogen activation in pancreatic acinar cells by influencing the function of autophagic lysosomes." Oncotarget9.13 (2018): 10868.

      Wen, Jiexia, et al. "Essential role of Na+/Ca2+ exchanger 1 in smoking-induced growth and migration of esophageal squamous cell carcinoma." Oncotarget 7.39 (2016): 63816.

      Yang, Chun‐He, et al. "Melatonin promotes secondary hair follicle development of early post‐natal cashmere goat and improves cashmere quantity and quality by enhancing antioxidant capacity and suppressing apoptosis." Journal of pineal research (2019): e12569.

      Zhang, Bo, Kun Ma, and Baixiang Li. "Inflammatory reaction regulated by microglia plays a role in atrazine-induced dopaminergic neuron degeneration in the substantia nigra." The Journal of toxicological sciences 40.4 (2015): 437-450.

      Zhuang, Hao, et al. "Glycine decarboxylase induces autophagy and is downregulated by miRNA-30d-5p in hepatocellular carcinoma." Cell death & disease 10.3 (2019).

      艾丽雅, et al. "蒙医不同针刺法对抑郁大鼠前额叶单胺类神经递质及 PKA/CREB/BDNF 通路的影响." 中国医药导报 16.8: 9-12.

      Feng, Wei, et al. "ERK/Drp1‐dependent mitochondrial fission contributes to HMGB1‐induced autophagy in pulmonary arterial hypertension." Cell proliferation 54.6 (2021): e13048.

      Wang, Yue, et al. "Fetal exposure to dichloroacetic acid and impaired cognitive function in the adulthood." Brain and Behavior 10.10 (2020): e01801.

      Kang, Tze Zhen Evangeline, et al. "The elevated transcription of ADAM19 by the oncohistone H2BE76K contributes to oncogenic properties in breast cancer." Journal of Biological Chemistry 296 (2021).

      Wang, Ji-Sheng, et al. "Effect of leech-centipede medicine on improving erectile function in DIED rats via PKC signalling pathway-related molecules." Journal of Ethnopharmacology267 (2021): 113463.

      Zhang, Ye, et al. "Fasciculation and Elongation Protein Zeta-1 Expression in Reactive Astrocytes in a Rat Model of Frontal Lobe Injury." Journal of Neuropathology & Experimental Neurology 79.2 (2020): 194-208.

      Bai, L., Kong, M., Duan, Z. et al. M2-like macrophages exert hepatoprotection in acute-on-chronic liver failure through inhibiting necroptosis-S100A9-necroinflammation axis. Cell Death Dis 12, 93 (2021). 

      Lv, Yajing, et al. "Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway." PLoS biology 18.11 (2020): e3000872.

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      Lv, Tianbao, et al. "Dipotassium glycyrrhizinate relieves leptospira-induced nephritis in vitro and in vivo." Microbial Pathogenesis 152 (2021): 104770.

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      Wang, Jisheng, et al. "Biological network model of effect of chronic intermittent hypoxia on spermatogenesis in rats." Medical Science Monitor: International Medical Journal of Experimental and Clinical Research 26 (2020): e925579-1.

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      Zeng, Yu, et al. "miR-150-5p mediates extravillous trophoblast cell migration and angiogenesis functions by regulating VEGF and MMP9." Placenta 93 (2020): 94-100.

      Li, Yujun, et al. "The inflammation and reactive oxygen species regulated by Nrf2 and NF-κB signaling pathways in 630-nm light-emitting diode irradiation treated THP-1 monocytes/macrophages." Lasers in Medical Science (2020): 1-9.

      Qianqian, Liu, Ding Shengkai, and Shang Yazhen. "Effect of Scutellaria Baicalensis Georgi Stems and Leaves Flavonoids on Multisite Phosphorylation of tau Protein Induced by Composited Aβ in Rats."

      Geng, Dong-Hui, et al. "Peptides YYGGEGSSSEQG and SESEM Inhibit TNF-α-Induced Smooth Muscle Cells Proliferation and Migration Through Their Bindings to TNF-α Receptor." International Journal of Peptide Research and Therapeutics 27.1 (2021): 405-411.

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      Chen, Jugang, et al. "Modulation of activated astrocytes in the hypothalamus paraventricular nucleus to prevent ventricular arrhythmia complicating acute myocardial infarction." International journal of cardiology 308 (2020): 33-41.

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      Ge, Pan, et al. "Rno_circRNA_016194 might be involved in the testicular injury induced by long-term formaldehyde exposure via rno-miR-449a-5p mediated Atg4b activation." Food and Chemical Toxicology (2021): 112409.

      Cong, Li, et al. "7-Difluoromethoxy-5, 4′-dimethoxy-genistein attenuates macrophages apoptosis to promote plaque stability via TIPE2/TLR4 axis in high fat diet-fed ApoE−/− mice." International Immunopharmacology 96 (2021): 107477.

      • Products Images
      • Wang, Yue, et al. "Fetal exposure to dichloroacetic acid and impaired cognitive function in the adulthood." Brain and Behavior 10.10 (2020): e01801.
      • Kang, Tze Zhen Evangeline, et al. "The elevated transcription of ADAM19 by the oncohistone H2BE76K contributes to oncogenic properties in breast cancer." Journal of Biological Chemistry 296 (2021).
      • Zhuang, Hao, et al. "Glycine decarboxylase induces autophagy and is downregulated by miRNA-30d-5p in hepatocellular carcinoma." Cell death & disease 10.3 (2019).
      • Bai, Jingchao, et al. "HSP 90 inhibitor AUY 922 can reverse Fulvestrant induced feedback reaction in human breast cancer cells." Cancer science 108.6 (2017): 1177-1184.
      • Tang, Qiusha, et al. "Combination of PEI-Mn0. 5Zn0. 5Fe2O4 nanoparticles and pHsp 70-HSV-TK/GCV with magnet-induced heating for treatment of hepatoma." International journal of nanomedicine 10 (2015): 7129.
      • Wang, Wu M., et al. "MicroRNA-182 regulates neurite outgrowth involving the PTEN/AKT pathway." Frontiers in cellular neuroscience 11 (2017): 96.
      • Liu, Chang, et al. "Mesenchymal Stem Cells Promote the Osteogenesis in Collagen-Induced Arthritic Mice through the Inhibition of TNF-α." Stem cells international 2018 (2018).
      • Tao, Yuquan, et al. "Huaier Augmented the Chemotherapeutic Sensitivity of Oxaliplatin via Downregulation of YAP in Hepatocellular Carcinoma." Journal of Cancer 9.21 (2018): 3962.
      • Liu, Yanmei, et al. "Cancer Stem Cells are Regulated by STAT3 Signalling in Wilms Tumour." Journal of Cancer 9.8 (2018): 1486.
      • Xu, Yingming, et al. "The spinal NR2BR/ERK2 pathway as a target for the central sensitization of collagen-induced arthritis pain." PloS one 13.7 (2018): e0201021.
      • Wang, Jun-Jie, et al. "Neuroprotective effect of Notch pathway inhibitor DAPT against focal cerebral ischemia/reperfusion 3 hours before model establishment." Neural regeneration research 14.3 (2019): 452.
      • Wen, Jiexia, et al. "Essential role of Na+/Ca2+ exchanger 1 in smoking-induced growth and migration of esophageal squamous cell carcinoma." Oncotarget 7.39 (2016): 63816.
      • Song, Zonggong, et al. "miR-352 participates in the regulation of trypsinogen activation in pancreatic acinar cells by influencing the function of autophagic lysosomes." Oncotarget9.13 (2018): 10868.
      • Immunofluorescence analysis of Hela cell. 1,Cyclin A Polyclonal Antibody(red) was diluted at 1:200(4° overnight). β-actin Monoclonal Antibody(5B7)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
      • Western blot analysis of 1 HEK293 2 SW480 3 HEPG2 4 MCF-7 5 mouse brain 6 Rat brain 7 Hela 8 A549 lysates, primary antibody was diluted at 1:5000, 4° over night, secondary antibody(cat: RS23910 was diluted at 1:10000, 37° 1hour.
      • Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,β-actin Monoclonal Antibody(5B7) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,β-actin Monoclonal Antibody(5B7) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,β-actin Monoclonal Antibody(5B7) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Western blot analysis of Hela (1) Rat brain (2) Mouse brain (3) Chicken lung (4) and Rabbit testis (5), diluted at 1:10000.
      • IHC Staining of Human ovary tissue, diluted at 1:200