β-actin Monoclonal Antibody(5B7)

    • Catalog No.:YM3028
    • Applications:IF,WB,IHC-p
    • Reactivity:Human,Rat,Mouse,Mk,Dg,Ch,Hamster,Rb,Insect
    • Description
    • Gene Name:
    • ACTB
    • Protein Name:
    • Actin cytoplasmic 1
    • Human Gene Id:
    • 60
    • Human Swiss Prot No:
    • P60709
    • Mouse Swiss Prot No:
    • P60710
    • Immunogen:
    • Synthetic Peptide of β-actin
    • Specificity:
    • The antibody detects endogenous β-actin protein.
    • Formulation:
    • PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
    • Source:
    • Mouse
    • Dilution:
    • IF: 1:50-200 WB: 1:5000 IHC: 1:200
    • Purification:
    • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
    • Concentration:
    • 1 mg/ml
    • Storage Stability:
    • -20°C/1 year
    • Other Name:
    • ACTB; Actin, cytoplasmic 1; Beta-actin
    • MolecularWeight(Da):
    • 41737
    • Observed Band(KD):
    • 43
    • Background:
    • β-Actin (gene name ACTB), a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. β-actin is a major constituent of the contractile apparatus, which is usually used as a loading control, for among others, the integrity of cells, protein degradation, in PCR and Western blotting. Its molecular weight is approximately 43 kDa.
    • Function:
    • cell morphogenesis, cell morphogenesis involved in differentiation, cell motion, axonogenesis, respo
    • Products Images
    • Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,β-actin Monoclonal Antibody(5B7) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    • Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,β-actin Monoclonal Antibody(5B7) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    • Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,β-actin Monoclonal Antibody(5B7) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    • Western blot analysis of Hela (1) Rat brain (2) Mouse brain (3) Chicken lung (4) and Rabbit testis (5), diluted at 1:10000.
    • IHC Staining of Human ovary tissue, diluted at 1:200