β-actin (PTR2364) mouse mAb
- Catalog No.:YM3028
- Applications:WB;IF;ELISA
- Reactivity:Human;Mouse;Rat;
- Target:
- Actin β
- Fields:
- >>Rap1 signaling pathway;>>Phagosome;>>Apoptosis;>>Hippo signaling pathway;>>Focal adhesion;>>Adherens junction;>>Tight junction;>>Platelet activation;>>Neutrophil extracellular trap formation;>>Leukocyte transendothelial migration;>>Thermogenesis;>>Regulation of actin cytoskeleton;>>Thyroid hormone signaling pathway;>>Oxytocin signaling pathway;>>Gastric acid secretion;>>Amyotrophic lateral sclerosis;>>Bacterial invasion of epithelial cells;>>Vibrio cholerae infection;>>Pathogenic Escherichia coli infection;>>Shigellosis;>>Salmonella infection;>>Yersinia infection;>>Influenza A;>>Proteoglycans in cancer;>>Hepatocellular carcinoma;>>Hypertrophic cardiomyopathy;>>Arrhythmogenic right ventricular cardiomyopathy;>>Dilated cardiomyopathy;>>Viral myocarditis;>>Fluid shear stress and atherosclerosis
- Gene Name:
- ACTB
- Protein Name:
- Actin cytoplasmic 1
- Human Gene Id:
- 60
- Human Swiss Prot No:
- P60709
- Mouse Gene Id:
- 11461
- Mouse Swiss Prot No:
- P60710
- Rat Gene Id:
- 81822
- Rat Swiss Prot No:
- P60711
- Immunogen:
- Synthesized peptide derived from human protein.AA range: 1-100
- Specificity:
- This antibody detects endogenous levels of β-actin protein.
- Formulation:
- PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
- Source:
- Mouse, Monoclonal/IgG1, kappa
- Dilution:
- WB 1:1000-5000. IF 1:100-500. ELISA 1:5000-50000
- Purification:
- Protein G
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- ACTB;Actin; cytoplasmic 1;Beta-actin;Actin β;β-Actin;Beta actin;B actin;actin Beta;
- Molecular Weight(Da):
- 42kD
- Observed Band(KD):
- 42kD
- Background:
- This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008],
- Function:
- disease:Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.,function:Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.,miscellaneous:In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.,similarity:Belongs to the
- Subcellular Location:
- Cytoplasmic
- Expression:
- B-cell lymphoma,Brain,Cajal-Retzius cell,Eye,Fetal brain cortex,Foreskin,Hepatocellular car
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Experimental and therapeutic medicine 17.3 (2019): 2349-2358.
7-Difluoromethoxy-5, 4′-dimethoxy-genistein attenuates macrophages apoptosis to promote plaque stability via TIPE2/TLR4 axis in high fat diet-fed ApoE−/− mice." International Immunopharmacology 96 (2021): 107477.
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Neuroprotective effect of Notch pathway inhibitor DAPT against focal cerebral ischemia/reperfusion 3 hours before model establishment. Neural Regeneration Research Neural Regen Res. 2019 Mar; 14(3): 452–461 WB Mouse 1:4000 Brain tissue
The permeability characteristics and interaction of the main components from Zhizi Bopi decoction in the MDCK cell model. XENOBIOTICA Xenobiotica. 2016;46(8):733-742 WB Dog Madin-Darby canine kidney (MDCK) cell
The elevated transcription of ADAM19 by the oncohistone H2BE76K contributes to oncogenic properties in breast cancer. JOURNAL OF BIOLOGICAL CHEMISTRY 2021 Jan;296: WB Human 293T cell,MDA-MB-231 cell
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Low LINC02147 expression promotes the malignant progression of oral submucous fibrosis BMC Oral Health Wenjie Li WB Human
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Deoxynivalenol damages the intestinal barrier and biota of the broiler chickens BMC Veterinary Research Fan Ruiwen WB Chicken
Polysaccharides from Tetrastigma Hemsleyanum Diels et Gilg attenuate LPS-induced acute lung injury by modulating TLR4/COX-2/NF-κB signaling pathway BIOMEDICINE & PHARMACOTHERAPY Bixu Wang WB Mouse, Human
Mitochondrial-Targeting Vitamin B3 Ameliorates the Phenotypes of Parkinson's Disease In Vitro and In Vivo by Restoring Mitochondrial Function Advanced Therapeutics Hua Bai WB Human
miRNA-331-3p Affects the Proliferation, Metastasis, and Invasion of Osteosarcoma through SOCS1/JAK2/STAT3 Journal of Oncology Bing Wu WB Mouse
Activation of Autophagy Induces Monocrotaline-Induced Pulmonary Arterial Hypertension by FOXM1-Mediated FAK Phosphorylation LUNG Shaojun Li WB Rat
Distribution characteristics and regulation of amino acids and fatty acids in muscle and adipose tissues of sheep grown in natural grazing environment ANIMAL SCIENCE JOURNAL Jinling Cao WB Sheep
Functional and metabolic alterations of gut microbiota in children with new-onset type 1 diabetes
Suppressing Mesenchymal Stromal Cell Ferroptosis Via Targeting a Metabolism-Epigenetics Axis Corrects their Poor Retention and Insufficient Healing Benefits in the Injured Liver Milieu Advanced Science Fuyang Zhang WB Human,Rat Mesenchymal stromal/stem cells (MSCs),Adipose-derived MSCs (ADSCs)
Immunoproteasome subunit β5i promotes perifascicular muscle atrophy in dermatomyositis by upregulating RIG-I RMD Open Qinglin Peng WB Human human skeletal muscle myoblasts (HSMMs),myoblast
The novel hepatoprotective mechanisms of silibinin-phospholipid complex against d-GalN/LPS-induced acute liver injury INTERNATIONAL IMMUNOPHARMACOLOGY Yu Chen WB Mouse liver tissues
Preliminary Structural Characterization of Selenium Nanoparticle Composites Modified by Astragalus Polysaccharide and the Cytotoxicity Mechanism on Liver Cancer Cells MOLECULES Xiaoyu Jia WB Human HepG2 cell
Postsurgical wound management and prevention of triple-negative breast cancer recurrence with a pryoptosis-inducing, photopolymerizable hydrogel JOURNAL OF CONTROLLED RELEASE Sanjun Shi WB Mouse 4 T1 cell
A Liquid–Liquid Phase Separation-Related Index Associate with Biochemical Recurrence and Tumor Immune Environment of Prostate Cancer Patients INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Ning Xu WB Human prostate cancer (PCa)cell
Clinopodium chinense Kuntze ameliorates dextran sulfate sodium-induced ulcerative colitis in mice by reducing systematic inflammation and regulating metabolism JOURNAL OF ETHNOPHARMACOLOGY Dali Meng WB Mouse colon RAW264.7 cell
Euphohelides A–C, ent-Abietane-Type Norditerpene Lactones from Euphorbia helioscopia and Their Anti-Inflammatory Activities JOURNAL OF NATURAL PRODUCTS Kun Gao WB Mouse RAW264.7 cell
Local analgesia of electroacupuncture is mediated by the recruitment of neutrophils and released β-endorphins PAIN Wang, Xiao-Yu WB Rat inflammatory tissue
Effects and mechanism of Compound Qidan Formula on rats with HFpEF induced by hypertension and diabetes mellitus based on Ang Ⅱ/TGF-β1/Smads signaling pathway JOURNAL OF ETHNOPHARMACOLOGY Dazhuo Shi WB Rat myocardial tissue
MAOA suppresses the growth of gastric cancer by interacting with NDRG1 and regulating the Warburg effect through the PI3K/AKT/mTOR pathway. Li Jun WB Human 1:5000 BGC-823 cell,HGC-27 cell
Naringenin prevents NAFLD in the diet-induced C57BL/6J obesity model by regulating the intestinal barrier function and microbiota. Yifei Bian WB Mouse 1:2000 colon tissue
Cathepsin B mediates the lysosomal-mitochondrial apoptosis pathway in arsenic-induced microglial cell injury. Baofei Sun WB Mouse 1:5000 BV2 cell
Simulated microgravity altered the proliferation, apoptosis, and extracellular matrix formation of L929 fibroblasts and the transforming growth factor-β1/Smad3 signaling pathway. Yan Cui WB Mouse L929 cell
HMGB1-induced activation of ER stress contributes to pulmonary artery hypertension in vitro and in vivo. Manxiang Li WB Rat lung tissue
Y-box binding protein 1 promotes chromatin accessibility to aggravate liver fibrosis. Xinyan Zhu WB Mouse,Human 1:5000 liver tissue LX-2 cell,hepatic stellate cell
Mesoporous polydopamine based biominetic nanodrug ameliorates liver fibrosis via antioxidation and TGF-β/SMADS pathway. Fei Liu WB Mouse liver
The gut metabolite 3-hydroxyphenylacetic acid rejuvenates spermatogenic dysfunction in aged mice through GPX4-mediated ferroptosis. Microbiome Zhe Zhang WB Mouse 1:3000 testis tissues GC-2 cell
Role of Txnrd3 in NiCl2-induced kidney cell apoptosis in mice: Potential therapeutic effect of melatonin. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Ziwei Zhang WB Mouse 1:1500 kidney
Tanshinone ⅡA participates in the treatment of endometriosis by regulating adhesion, invasion, angiogenesis and inhibition of PI3K/Akt/mTOR signaling pathway. Molecular Medicine Reports Xin Gong WB Rat 1:5000 endometria
ERRα promotes glycolytic metabolism and targets the NLRP3/caspase-1/GSDMD pathway to regulate pyroptosis in endometrial cancer. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH Pengming Sun WB Human 1:2000 KLE cell,HEC-1A cell
Alternate-day fasting ameliorated anxiety-like behavior in high-fat diet-induced obese mice JOURNAL OF NUTRITIONAL BIOCHEMISTRY Huijuan Hu WB Mouse 1:1000 hippocampal tissue
Astrocyte and L-lactate in the anterior cingulate cortex modulate schema memory and neuronal mitochondrial biogenesis eLife Mastura Akter WB Rat 1:5000 brain tissue
Downhill running and caloric restriction attenuate insulin resistance associated skeletal muscle atrophy via the promotion of M2-like macrophages through TRIB3-AKT pathway FREE RADICAL BIOLOGY AND MEDICINE Wei Luo WB Mouse 1:5000 C2C12 cell,RAW264.7 cell
Inhibición de la Proliferación, Migración e Invasión de Fibroblastos Queloides por miR-183-5p Mediante la Regulación Negativa de EGR1 INTERNATIONAL JOURNAL OF MORPHOLOGY Mingxi Li WB Human 1:5000 Keloid fibroblasts (KFs)
Study of the Mechanism of Astragali Radix in Treating Type 2 Diabetes Mellitus and Its Renal Protection Based on Enzyme Activity, Network Pharmacology, and Experimental Verification MOLECULES Chunnan Li WB Mouse renal tissue
Sunitinib induces cardiotoxicity through modulating oxidative stress and Nrf2-dependent ferroptosis in vitro and in vivo CHEMICO-BIOLOGICAL INTERACTIONS Dongjie Li WB Mouse,Rat heart tissue H9c2 cell
Dietary methionine supplementation during the estrous cycle improves follicular development and estrogen synthesis in rats Food & Function Guangxin Yang WB Rat 1:5000 Rat ovarian granulosa cells (rGCs)
Extracellular CIRP induces abnormal activation of fibroblast-like synoviocytes from patients with RA via the TLR4-mediated HDAC3 pathways INTERNATIONAL IMMUNOPHARMACOLOGY Feng Yao WB Human 1:5000 fibroblast-like synoviocytes (FLSs)
Huangqi Baihe Granules alleviate hypobaric hypoxia-induced acute lung injury in rats by suppressing oxidative stress and the TLR4/NF-κB/NLRP3 inflammatory pathway JOURNAL OF ETHNOPHARMACOLOGY Yuanding Zeng WB Rat 1:10000 lung tissue
Galectin from Trichinella spiralis alleviates DSS-induced colitis in mice by regulating the intestinal microbiota VETERINARY RESEARCH Li Jianqing WB Mouse 1:1000 colon tissue
Lamin B1: a novel biomarker in adult and pediatric adrenocortical carcinoma ENDOCRINE-RELATED CANCER Yihao Chen WB Human 1:5000 SW13 cell,H295R cell
Uncovering the Multitarget Therapeutic Mechanism of Tong-Xie-Yao-Fang on Irritable Bowel Syndrome JOURNAL OF FOOD QUALITY Xiangxue Ma WB Rat 1:5000 colonic tissue
Roles of ferroptosis in type 1 diabetes induced spermatogenic dysfunction FREE RADICAL BIOLOGY AND MEDICINE Yalei Cao WB Mouse 1:3000 testis tissue GC-2 cell
Inhibition of PI3K/AKT signaling pathway prevents blood-induced heterotopic ossification of the injured tendon Journal of Orthopaedic Translation Xuri Chen WB Mouse 1:1000 tendon stem/progenitor cells (TSPCs)
Nrf2-mediated redox balance alleviates LPS-induced vascular endothelial cell inflammation by inhibiting endothelial cell ferroptosis Scientific Reports Hou Huimin WB Human 1:1000 Human umbilical vein endothelial cells (HUVEC)
Blocking Ubiquitin-Specific Protease 7 Induces Ferroptosis in Gastric Cancer via Targeting Stearoyl-CoA Desaturase Advanced Science Xiaoqing Guan WB Mouse,Human MGC803-luc cell-Xenograft,MKN1-luc cell-Xenograft MGC803 cell,HGC27 cell,AGS cell,AZ521 cell
miR-375-3p targets YWHAB to attenuate intestine injury in neonatal necrotizing enterocolitis PEDIATRIC SURGERY INTERNATIONAL Nan Li WB Mouse,Human,Rat 1:5000 intestinal tissue IEC-6 cell
A novel multifunctional microneedle patch for synergistic photothermal- gas therapy against maxillofacial malignant melanoma and associated skin defects JOURNAL OF NANOBIOTECHNOLOGY Shaojie Dong WB Mouse novel multifunctional sodium nitroprusside and Fe2+ ions loaded microneedles (SNP-Fe@MNs)
Exocarpium Citri Grandis ameliorates LPS-induced acute lung injury by suppressing inflammation, NLRP3 inflammasome, and ferroptosis JOURNAL OF ETHNOPHARMACOLOGY Zaibin Xu WB Mouse Lung tissue RAW264.7 cell
A macromolecular Gleditsia Saponin induces apoptosis of leukemia cells through Fas-mediated death receptor pathway and mitochondrial pathway Food Bioscience Wen-jie Ding WB Mouse,Human 1:5000 K562 cell,L1210 cell
Gastrodin alleviates the deterioration of depressive-like behavior and glucolipid metabolism promoted by chronic stress in type 2 diabetic mice EUROPEAN JOURNAL OF PHARMACOLOGY Canye Li WB Mouse 1:5000 liver tissue
Machine-learning developed an iron, copper, and sulfur-metabolism associated signature predicts lung adenocarcinoma prognosis and therapy response RESPIRATORY RESEARCH Liangyu Zhang WB Human 1:1000 A549 cell, PC9 cell, H1975 cell
Danggui-Shaoyao San alleviates cognitive impairment via enhancing HIF-1α/EPO axis in vascular dementia rats JOURNAL OF ETHNOPHARMACOLOGY Yuan Ningning WB Rat 1:10000 hippocampus tissue
Fluoride exposure during puberty induces testicular impairment via ER stress-triggered apoptosis in mice FOOD AND CHEMICAL TOXICOLOGY Rong Wang WB Mouse testicular tissue
The involvement of oxidative stress and the TLR4/NF-κB/NLRP3 pathway in acute lung injury induced by high-altitude hypoxia IMMUNOBIOLOGY Wangjie Cao WB Rat lung tissue
Mussel-Derived and Bioclickable Peptide Mimic for Enhanced Interfacial Osseointegration via Synergistic Immunomodulation and Vascularized Bone Regeneration Advanced Science Wei Zhou WB Mouse,Rat femur tissue bone marrow mesenchymal stem cell(BMSC),RAW264.7 cell
PoRVA G9P[23] and G5P[7] infections differentially promote PEDV replication by reprogramming glutamine metabolism PLoS Pathogens Haixin Liu WB Pig IPEC-J2 cell
Sinensetin, a polymethoxyflavone from citrus fruits, ameliorates LPS-induced acute lung injury by suppressing Txnip/NLRP3/Caspase-1/GSDMD signaling-mediated inflammatory responses and pyroptosis Food & Function Zaibin Xu WB Mouse lung tissue RAW264.7 cell
POSTN promotes nucleus pulposus cell senescence and extracellular matrix metabolism via activing Wnt/β-catenin and NF-κB signal pathway in intervertebral disc degeneration CELLULAR SIGNALLING Daxue Zhu WB Human,Rat 1:5000 nucleus pulposus (NP) tissue nucleus pulposus cell
Low Expression of Selenoprotein S Induces Oxidative Damage in Cartilages JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY Yixin Cui WB Mouse 1:1000 ATDC5 chondrocytes
Keratinocyte-derived small extracellular vesicles delay diabetic wound healing by triggering fibroblasts autophagy ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY Xiaosi Hong WB Human Human primary foreskin fibroblasts
Machine learning-based autophagy-related prognostic signature for personalized risk stratification and therapeutic approaches in bladder cancer INTERNATIONAL IMMUNOPHARMACOLOGY Zhen Wang WB Human 1:5000 T24 cell,5637 cell
Neuroprotective effects of total phenolics from Hemerocallis citrina Baroni leaves through the PI3K/AKT pathway Frontiers in Pharmacology Jia Yanjun WB Rat PC12 cell
Weissella paramesenteroides NRIC1542 inhibits dextran sodium sulfate-induced colitis in mice through regulating gut microbiota and SIRT1/NF-κB signaling pathway FASEB JOURNAL Shuang Li WB Mouse 1:4000 colon tissue
MFN2-dependent mitochondrial dysfunction contributes to Relm-β-induced pulmonary arterial hypertension via USP18/Twist1/miR-214 pathway EUROPEAN JOURNAL OF PHARMACOLOGY Yan Wang WB Rat 1:1000 lung tissue Pulmonary arterial smooth muscle cell(PASMC)
Targeting Hypoxia and HIF1α in Triple-Negative Breast Cancer: New Insights from Gene Expression Profiling and Implications for Therapy Biology-Basel Delong Han WB Human 1:5000 MDA-MB-231 cell
Reversible control of kinase signaling through chemical-induced dephosphorylation Communications Biology Sun Ying WB Mouse Ba/F3 cell
Transcutaneous Auricular Vagus Stimulation Attenuates LPS-Induced Depression-Like Behavior by Regulating Central α7nAChR/JAK2 Signaling MOLECULAR NEUROBIOLOGY Wang Junying WB Rat 1:5000 brain tissue
Hypoxia-Induced Adaptations of Embryonic Fibroblasts: Implications for Developmental Processes Biology-Basel Zeyu Li WB Mouse mouse embryonic fibroblasts (MEFs)
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- Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-β-actin (PTR2364) antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: AGS Lane 2: A375 Lane 3: A431 Lane 4: A549 Lane 5: ACHN Lane 6: Daudi Lane 7: Du145 Lane 8: H69AR Lane 9: HACAT Lane 10: HepG2
- Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-β-actin (PTR2364) antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: HUVEC Lane 2:JAR Lane 3:Jurkat Lane 4:LN18 Lane 5:MCF7 Lane 6:MES-SA Lane 7:MG-63 Lane 8:MRC-5 Lane 9:NIH-3T3 Lane 10:OVCAR-3
- Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-β-actin (PTR2364) antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: PC-3 Lane 2: PC-12 Lane 3: Raji Lane 4: Ramos Lane 5: Saos-2 Lane 6: RAW264.7 Lane 7: SH-SY5Y Lane 8: SW480 Lane 9: T47D Lane 10: U2OS
- Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-β-actin (PTR2364) antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: U-87 MG Lane 2: Karpas-299 Lane 3: Hela Lane 4: HEK293 Lane 5: Mouse Lung Lane 6: mouse testis Lane 7: Rabbit brain
- Postsurgical wound management and prevention of triple-negative breast cancer recurrence with a pryoptosis-inducing, photopolymerizable hydrogel JOURNAL OF CONTROLLED RELEASE Sanjun Shi WB Mouse 4 T1 cell
- Wang, Yue, et al. "Fetal exposure to dichloroacetic acid and impaired cognitive function in the adulthood." Brain and Behavior 10.10 (2020): e01801.
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- Wang, Wu M., et al. "MicroRNA-182 regulates neurite outgrowth involving the PTEN/AKT pathway." Frontiers in cellular neuroscience 11 (2017): 96.
- Liu, Chang, et al. "Mesenchymal Stem Cells Promote the Osteogenesis in Collagen-Induced Arthritic Mice through the Inhibition of TNF-α." Stem cells international 2018 (2018).
- Tao, Yuquan, et al. "Huaier Augmented the Chemotherapeutic Sensitivity of Oxaliplatin via Downregulation of YAP in Hepatocellular Carcinoma." Journal of Cancer 9.21 (2018): 3962.
- Liu, Yanmei, et al. "Cancer Stem Cells are Regulated by STAT3 Signalling in Wilms Tumour." Journal of Cancer 9.8 (2018): 1486.
- Xu, Yingming, et al. "The spinal NR2BR/ERK2 pathway as a target for the central sensitization of collagen-induced arthritis pain." PloS one 13.7 (2018): e0201021.
- Wang, Jun-Jie, et al. "Neuroprotective effect of Notch pathway inhibitor DAPT against focal cerebral ischemia/reperfusion 3 hours before model establishment." Neural regeneration research 14.3 (2019): 452.
- Wen, Jiexia, et al. "Essential role of Na+/Ca2+ exchanger 1 in smoking-induced growth and migration of esophageal squamous cell carcinoma." Oncotarget 7.39 (2016): 63816.
- Song, Zonggong, et al. "miR-352 participates in the regulation of trypsinogen activation in pancreatic acinar cells by influencing the function of autophagic lysosomes." Oncotarget9.13 (2018): 10868.