GAPDH Monoclonal Antibody(2B8)

    • Catalog No.:YM3029
    • Applications:WB,IF,IHC-p
    • Reactivity:Human,Rat,Mouse,Mk,Dg,Ch,Hamster,Rb,Pig,sheep,Insect,Yeast
      • Gene Name:
      • GAPDH
      • Protein Name:
      • Glyceraldehyde-3-phosphate dehydrogenase
      • Human Gene Id:
      • 2597
      • Human Swiss Prot No:
      • P04406
      • Mouse Swiss Prot No:
      • P16858
      • Immunogen:
      • Synthetic Peptide of GAPDH
      • Specificity:
      • The antibody detects endogenous GAPDH protein.
      • Formulation:
      • PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
      • Source:
      • Mouse
      • Dilution:
      • WB: 1:5000 IHC: 1:200 IF 1:200
      • Purification:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH
      • Observed Band(KD):
      • 37
      • Background:
      • glyceraldehyde-3-phosphate dehydrogenase(GAPDH) Homo sapiens This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferri
      • Function:
      • catalytic activity:D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.,function:Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.,online information:Glyceraldehyde 3-phosphate dehydrogenase entry,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1.,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.,PTM:Reversible S-nitrosylation of Cys-152 inhibits enzymatic activity and increases endogenous ADP-ribosylation, which inhibits the enzyme in a non-reversible manner. The latter modification is more likely to be a pathophysiological event associated with inhibition of gluconeogenesis.,sequence caution:Differs quite extensively.,similarity:Belongs to the glyceraldehyde-3-phosphate dehydrogenase fami
      • Subcellular Location:
      • nucleus,cytoplasm,lipid particle,cytosol,plasma membrane,microtubule cytoskeleton,membrane,intracellular ribonucleoprotein complex,extracellular matrix,nuclear membrane,vesicle,intracellular membrane-bounded orga
      • Expression:
      • Astrocytoma,Brain,Cajal-Retzius cell,Colon adenocarcinoma,Epitheliu

      Zhi, Hui, et al. "Decarbromodiphenyl ether (BDE-209) promotes monocyte–endothelial adhesion in cultured human aortic endothelial cells through upregulating intercellular adhesion molecule-1." Environmental research 169 (2019): 62-71.
      Catalog No.:YM3029

      Liang, Xiaofang, et al. "Growth and feed intake regulation responses to anorexia, adaptation and fasting in Japanese seabss, Lateolabrax japonicas when fishmeal is totally replaced by plant protein." Aquaculture 498 (2019): 528-538.
      Catalog No.:YM3029

      Jing, Shen, et al. "Imaging Potential Evaluation of Fab Derived from the Anti-EGFRvIII Monoclonal Antibody 4G1." Radiation research (2018).
      Catalog No.:ym3029

      Gong, Ningning, et al. "Adipokine expression and endothelial function in subclinical hypothyroidism rats." Endocrine connections 7.2 (2018): 295-304.
      Catalog No.:ym3029

      Wu, Shiyong, et al. "Trimetazidine protects against myocardial ischemia/reperfusion injury by inhibiting excessive autophagy." Journal of Molecular Medicine 96.8 (2018): 791-806.
      Catalog No.:ym3029

      Zhou, Xi-Dan, Dong-Dong Shi, and Zhang-Jin Zhang. "Antidepressant and anxiolytic effects of the proprietary Chinese medicine Shexiang Baoxin pill in mice with chronic unpredictable mild stress." Journal of food and drug analysis(2018).
      Catalog No.:ym3029

      Zhi, Hui, et al. "Decabromodiphenyl ether (BDE-209) enhances foam cell formation in human macrophages via augmenting Toll-like receptor 4-dependent lipid uptake." Food and Chemical Toxicology 121 (2018): 367-373.
      Catalog No.:ym3029

      Li, Gang, et al. "SOST, an LNGFR target, inhibits the osteogenic differentiation of rat ectomesenchymal stem cells." Cell proliferation 51.2 (2018): e12412.
      Catalog No.:ym3029

      Chen, Luchao, et al. "Effects of purified Omphalia lapidescens protein on metastasis, cell cycle, apoptosis and the JAK-STAT signaling pathway in SGC-7901 human gastric cells." Oncology letters 15.4 (2018): 4161-4170.
      Catalog No.:YM3029

      Niu, Yan-Long, et al. "Long-term strength training causes a downregulation of nephrin in the slit diaphragm of kidneys in rats." Int J Clin Exp Med 11.7 (2018): 6965-6972.
      Catalog No.:YM3029

      Du, Shaobo, et al. "Lycium barbarum Polysaccharides Protect Rat Corneal Epithelial Cells against Ultraviolet B-Induced Apoptosis by Attenuating the Mitochondrial Pathway and Inhibiting JNK Phosphorylation." BioMed research international 2017 (2017).
      Catalog No.:ym3029

      Yang, Deng-Hua, et al. "The histone deacetylase inhibitor sodium butyrate protects against noise-induced hearing loss in Guinea pigs." Neuroscience letters 660 (2017): 140-146.
      Catalog No.:ym3029

      Wei, Yuzhen, et al. "CD4+ CD25+ GARP+ regulatory T cells display a compromised suppressive function in patients with dilated cardiomyopathy." Immunology 151.3 (2017): 291-303.
      Catalog No.:ym3029

      Yu, Qianqian, et al. "Comparative proteomics to reveal muscle-specific beef color stability of Holstein cattle during post-mortem storage." Food chemistry 229 (2017): 769-778.
      Catalog No.:YM3029

      Liu, Xuemei, et al. "improvement in cerebral ischemia–reperfusion injury through the Tlr4/nf-κb pathway after Kudiezi injection in rats." Life sciences 191 (2017): 132-140.
      Catalog No.:YM3029

      Jia, Yan-Fei, et al. "Expression of kisspeptin-GnRH system is down-regulated in hypothalamic arcuate nucleus of male rats with high-fat-diet." INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 10.5 (2017): 6099-6107.
      Catalog No.:YM3029

      Wang, Yan, et al. "Suppressive effect of β, β-dimethylacryloyl alkannin on activated dendritic cells in psoriasis by the TLR7/8 pathway." International immunopharmacology 40 (2016): 410-418.
      Catalog No.:ym3029

      Zeng, Jin, et al. "Regulation of intrinsic apoptosis in cycloheximide-treated macrophages by the Sichuan human strain of Chinese Leishmania isolates." Acta tropica 153 (2016): 101-110.
      Catalog No.:ym3029

      Zhang, Lei, et al. "Increased α-actinin-2 expression in the atrial myocardium of patients with atrial fibrillation related to rheumatic heart disease." Cardiology 135.3 (2016): 151-159.
      Catalog No.:ym3029

      Zhang, Chengke, et al. "Induction of size-dependent breakdown of blood-milk barrier in lactating mice by TiO2 nanoparticles." PloS one 10.4 (2015): e0122591.
      Catalog No.:ym3029

      Wang, Zeming, et al. "Effects of icariin on the regulation of the OPG‑RANKL‑RANK system are mediated through the MAPK pathways in IL‑1β‑stimulated human SW1353 chondrosarcoma cells." International journal of molecular medicine 34.6 (2014): 1720-1726.
      Catalog No.:ym3029

      Liu, Wei-hua, et al. "Low-dose decitabine induces MAGE-A expression and inhibits invasion via suppression of NF-κB2 and MMP2 in Eca109 cells." Biomedicine & Pharmacotherapy 68.6 (2014): 745-750.
      Catalog No.:ym3029

      • Products Images
      • Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1,GAPDH Monoclonal Antibody(2B8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,GAPDH Monoclonal Antibody(2B8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,GAPDH Monoclonal Antibody(2B8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunofluorescence analysis of Human-colon tissue. 1,GAPDH Monoclonal Antibody(2B8)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
      • Immunofluorescence analysis of Mouse-liver tissue. 1,GAPDH Monoclonal Antibody(2B8)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
      • Western blot analysis of Hela (1), Rat brain (2), Rabbit Muscle (3), Sheep Muscle (4), and Mouse brain (5), diluted at 1:10000.
      • The picture was kindly provided by our customer
      • The picture was kindly provided by our customer
      • The picture was kindly provided by our customer