GAPDH Monoclonal Antibody(2B8)

    • Catalog No.:YM3029
    • Applications:WB,IF,IHC-p
    • Reactivity:Human,Rat,Mouse,Mk,Dg,Ch,Hamster,Rb,Pig,sheep,Insect,Yeast
    • Description
    • Gene Name:
    • GAPDH
    • Protein Name:
    • Glyceraldehyde-3-phosphate dehydrogenase
    • Human Gene Id:
    • 2597
    • Human Swiss Prot No:
    • P04406
    • Mouse Swiss Prot No:
    • P16858
    • Immunogen:
    • Synthetic Peptide of GAPDH
    • Specificity:
    • The antibody detects endogenous GAPDH protein.
    • Formulation:
    • PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
    • Source:
    • Mouse
    • Dilution:
    • WB: 1:5000 IHC: 1:200 IF 1:200
    • Purification:
    • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
    • Storage Stability:
    • -20°C/1 year
    • Other Name:
    • GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH
    • Observed Band(KD):
    • 37
    • Background:
    • Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.
    • Function:
    • monosaccharide metabolic process, glucose metabolic process, glucose catabolic process, generation o
    • Products Images
    • Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1,GAPDH Monoclonal Antibody(2B8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    • Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,GAPDH Monoclonal Antibody(2B8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    • Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,GAPDH Monoclonal Antibody(2B8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    • Immunofluorescence analysis of Human-colon tissue. 1,GAPDH Monoclonal Antibody(2B8)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    • Immunofluorescence analysis of Mouse-liver tissue. 1,GAPDH Monoclonal Antibody(2B8)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    • Western blot analysis of Hela (1), Rat brain (2), Rabbit Muscle (3), Sheep Muscle (4), and Mouse brain (5), diluted at 1:10000.
    • The picture was kindly provided by our customer
    • The picture was kindly provided by our customer
    • The picture was kindly provided by our customer