RIP rabbit pAb
- Catalog No.:YT7091
- Applications:WB;ELISA;IHC
- Reactivity:Human;Mouse;Rat
- Target:
- RIP
- Gene Name:
- RPAIN RIP
- Protein Name:
- RIP
- Human Gene Id:
- 84268
- Human Swiss Prot No:
- Q86UA6
- Mouse Gene Id:
- 69723
- Mouse Swiss Prot No:
- Q9CWY9
- Rat Gene Id:
- 287463
- Rat Swiss Prot No:
- Q4G2Y1
- Immunogen:
- Synthesized peptide derived from human RIP AA range: 117-167
- Specificity:
- This antibody detects endogenous levels of RIP at Human/Mouse/Rat
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Molecular Weight(Da):
- 24kD
- Function:
- function:Mediates the import of RPA complex into the nucleus, possibly via some interaction with importin beta. Isoform 2 is sumoylated and mediates the localization of RPA complex into the PML body of the nucleus, thereby participating in RPA function in DNA metabolism.,PTM:Isoform 2 is sumoylated. Sumoylation is required for localization in the nuclear PML body and transport of RPA complex in PML body. Upon UV irradiation and during S phase, it is desumoylated, releasing RPA complex that is translocated to sites of DNA damage. Sumoylation takes place at different Lys residues.,similarity:Contains 1 RIP-type zinc finger.,subcellular location:Only nuclear, localizes to PML body.,subunit:Interacts with the RPA1 subunit of RPA complex.,tissue specificity:Widely expressed. Expressed in pancreas, kidney, muscle, liver, lung, placenta, brain, heart, leukocytes, colon, intestine, ovary, testis
- Subcellular Location:
- [Isoform 1]: Cytoplasm. Nucleus.; [Isoform 2]: Nucleus, PML body.
- Expression:
- Widely expressed. Expressed in pancreas, kidney, muscle, liver, lung, placenta, brain, heart, leukocytes, colon, intestine, ovary, testis, prostate, thymus and spleen.
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- Antibody-FAQs
- Products Images
- Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°over night
- Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).