• Target：
• MCM7

• Fields：
• >>DNA replication;>>Cell cycle

• Gene Name：
• MCM7

• Protein Name：
• DNA replication licensing factor MCM7

• Human Gene Id：
• 4176

• Human Swiss Prot No：
• P33993

• Mouse Gene Id：
• 17220

• Mouse Swiss Prot No：
• Q61881

• Immunogen：
• The antiserum was produced against synthesized peptide derived from the Internal region of human MCM7. AA range:491-540

• Specificity：
• MCM7 Polyclonal Antibody detects endogenous levels of MCM7 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• MCM7;CDC47;MCM2;DNA replication licensing factor MCM7;CDC47 homolog;P1.1-MCM3

• Observed Band(KD)：
• 81kD

• Background：
• The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008],

• Function：
• function:Acts as a factor that allows the DNA to undergo a single round of replication per cell cycle. Required for DNA replication and cell proliferation. Required for S-phase checkpoint activation upon UV-induced damage.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the MCM family.,similarity:Contains 1 MCM domain.,subunit:Interacts with the ATR-ATRIP complex and with RAD17. Interacts with TIPIN.,

• Subcellular Location：
• Nucleus . Chromosome . Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses. .

• Expression：
• Brain,Cervix,Chronic myeloid leukemia cell,Lung,

The Catalytically Inactive Mutation of the Ubiquitin-Conjugating Enzyme CDC34 Affects its Stability and Cell Proliferation. PROTEIN JOURNAL Protein J. 2018 Apr;37(2):132-143 WB Human HEK293T cell, HeLa cell

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