Photomedin-1 Polyclonal Antibody

    • Catalog No.:YT3705
    • Applications:WB;IHC
    • Reactivity:Human;Mouse
      • Target:
      • Photomedin-1
      • Gene Name:
      • OLFML2A
      • Protein Name:
      • Olfactomedin-like protein 2A
      • Human Swiss Prot No:
      • Q68BL7
      • Mouse Swiss Prot No:
      • Q8BHP7
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human OLFML2A. AA range:311-360
      • Specificity:
      • Photomedin-1 Polyclonal Antibody detects endogenous levels of Photomedin-1 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500-2000;IHC 1:50-300
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • OLFML2A;Olfactomedin-like protein 2A;Photomedin-1
      • Observed Band(KD):
      • 73kD
      • Background:
      • PTM:May be cleaved at Lys-295 after secretion.,PTM:O-glycosylated but not N-glycosylated.,similarity:Contains 1 olfactomedin-like domain.,subunit:Homodimer. Binds to heparin and chondroitin sulfate E.,
      • Function:
      • PTM:May be cleaved at Lys-295 after secretion.,PTM:O-glycosylated but not N-glycosylated.,similarity:Contains 1 olfactomedin-like domain.,subunit:Homodimer. Binds to heparin and chondroitin sulfate E.,
      • Subcellular Location:
      • Secreted . Localizes to the podocyte major processes. Colocalized with the major process protein VIM throughout podocyte development. .
      • Expression:
      • In the kidney expressed only by podocytes, wherein they localize to major processes.
      • Products Images
      • Western Blot analysis of RAW264.7 cells using Photomedin-1 Polyclonal Antibody
      • Immunofluorescence analysis of A549 cells, using OLFML2A Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from RAW264.7 cells, using OLFML2A Antibody. The lane on the right is blocked with the synthesized peptide.
      • Immunohistochemical analysis of paraffin-embedded human meningioma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).