• Target：
• Ndfip1

• Gene Name：
• NDFIP1

• Protein Name：
• NEDD4 family-interacting protein 1

• Human Gene Id：
• 80762

• Human Swiss Prot No：
• Q9BT67

• Mouse Gene Id：
• 65113

• Mouse Swiss Prot No：
• Q8R0W6

• Rat Gene Id：
• 291609

• Rat Swiss Prot No：
• Q5U2S1

• Immunogen：
• Synthesized peptide derived from Ndfip1 . at AA range: 150-230

• Specificity：
• Ndfip1 Polyclonal Antibody detects endogenous levels of Ndfip1 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• NDFIP1;N4WBP5;PSEC0192;PSEC0223;NEDD4 family-interacting protein 1;Breast cancer-associated protein SGA-1M;NEDD4 WW domain-binding protein 5;Putative MAPK-activating protein PM13;Putative NF-kappa-B-activating protein 164;Putative

• Observed Band(KD)：
• 25kD

• Background：
• The protein encoded by this gene belongs to a small group of evolutionarily conserved proteins with three transmembrane domains. It is a potential target for ubiquitination by the Nedd4 family of proteins. This protein is thought to be part of a family of integral Golgi membrane proteins. [provided by RefSeq, Jul 2008],

• Function：
• developmental stage:Expressed in fetal brain, kidney and lung.,domain:The WW-binding motifs mediate interaction with NEDD4.,function:May play a role in Golgi structure maintenance.,PTM:Ubiquitinated by NEDD4; mono-, di- and polyubiquitinated forms are detected. Ubiquitination regulates its degradation.,subunit:Interacts with NEDD4. Interacts in vitro with NEDD4L, SR140, WWP2 and ITCH.,tissue specificity:Widely expressed. Higher levels are detected in cerebellum, pituitary, thalamus, kidney, liver, testis, salivary glands and placenta.,

• Subcellular Location：
• Endosome membrane ; Multi-pass membrane protein . Golgi apparatus membrane . Cell junction, synapse, synaptosome . Cell projection, dendrite . Secreted . Detected in exosomes and secreted via the exosomal pathway (PubMed:18819914).

• Expression：
• Widely expressed. Higher levels are detected in cerebellum, pituitary, thalamus, kidney, liver, testis, salivary glands and placenta. Also expressed in fetal brain, kidney and lung.

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