• Target：
• iPLA2γ

• Gene Name：
• PNPLA8

• Protein Name：
• Calcium-independent phospholipase A2-gamma

• Human Gene Id：
• 50640

• Human Swiss Prot No：
• Q9NP80

• Mouse Gene Id：
• 67452

• Mouse Swiss Prot No：
• Q8K1N1

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human PNPLA8. AA range:691-740

• Specificity：
• iPLA2γ Polyclonal Antibody detects endogenous levels of iPLA2γ protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500-2000;IHC 1:50-300

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• PNPLA8;IPLA22;IPLA2G;BM-043;Calcium-independent phospholipase A2-gamma;Intracellular membrane-associated calcium-independent phospholipase A2 gamma;iPLA2-gamma;PNPLA-gamma;Patatin-like phospholipase domain-containing protein 8;iPLA

• Observed Band(KD)：
• 88kD

• Background：
• This gene encodes a member of the patatin-like phospholipase domain containing protein family. Members of this family are phospholipases which catalyze the cleavage of fatty acids from membrane phospholipids. The product of this gene is a calcium-independent phospholipase. Mutations in this gene have been associated with mitochondrial myopathy with lactic acidosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2015],

• Function：
• catalytic activity:2-lysophosphatidylcholine + H(2)O = glycerophosphocholine + a carboxylate.,enzyme regulation:Inhibited by E-6-bromomethylene-3-1-naphthalenyl-2H-tetrahydropyran-2-one (BEL).,function:Calcium-independent phospholipase A2, which catalyzes the hydrolysis of the sn-2 position of glycerophospholipids, PtdSer and to a lower extent PtdCho. Cleaves membrane phospholipids.,similarity:Contains 1 patatin domain.,tissue specificity:Expressed in parenchymal tissues including heart, skeletal muscle, placenta, brain, liver and pancreas. Also expressed in bronchial epithelial cells and kidney. Highest expression is observed in skeletal muscle and heart.,

• Subcellular Location：
• Endoplasmic reticulum membrane ; Single-pass membrane protein . Mitochondrion membrane ; Single-pass membrane protein . Peroxisome membrane ; Single-pass membrane protein .

• Expression：
• Expressed in parenchymal tissues including heart, skeletal muscle, placenta, brain, liver and pancreas. Also expressed in bronchial epithelial cells and kidney. Highest expression is observed in skeletal muscle and heart.

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