Id4 Polyclonal Antibody

    • Catalog No.:YT2272
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • Id4
      • Fields:
      • >>TGF-beta signaling pathway;>>Signaling pathways regulating pluripotency of stem cells
      • Gene Name:
      • ID4
      • Protein Name:
      • DNA-binding protein inhibitor ID-4
      • Human Gene Id:
      • 3400
      • Human Swiss Prot No:
      • P47928
      • Mouse Swiss Prot No:
      • P41139
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human ID4. AA range:1-50
      • Specificity:
      • Id4 Polyclonal Antibody detects endogenous levels of Id4 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • ID4;BHLHB27;DNA-binding protein inhibitor ID-4;Class B basic helix-loop-helix protein 27;bHLHb27;Inhibitor of DNA binding 4
      • Observed Band(KD):
      • 24kD
      • Background:
      • This gene encodes a member of the inhibitor of DNA binding (ID) protein family. These proteins are basic helix-loop-helix transcription factors which can act as tumor suppressors but lack DNA binding activity. Consequently, the activity of the encoded protein depends on the protein binding partner. [provided by RefSeq, Dec 2011],
      • Function:
      • function:ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Heterodimer with other HLH proteins.,
      • Subcellular Location:
      • Nucleus.
      • Expression:
      • Abdominal adipose tissue,Uterus,
      • Products Images
      • Western Blot analysis of various cells using Id4 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
      • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
      • Western blot analysis of lysates from HepG2 cells, using ID4 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Western blot analysis of the lysates from HeLa cells using ID4 antibody.