Histamine H4 Receptor Polyclonal Antibody
- Catalog No.:YT2143
- Applications:WB;IF;ELISA
- Reactivity:Human;Rat;Mouse;
- Target:
- Histamine H4 Receptor
- Fields:
- >>Neuroactive ligand-receptor interaction
- Gene Name:
- HRH4
- Protein Name:
- Histamine H4 receptor
- Human Gene Id:
- 59340
- Human Swiss Prot No:
- Q9H3N8
- Mouse Swiss Prot No:
- Q91ZY2
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human HRH4. AA range:221-270
- Specificity:
- Histamine H4 Receptor Polyclonal Antibody detects endogenous levels of Histamine H4 Receptor protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- HRH4;GPCR105;Histamine H4 receptor;H4R;HH4R;AXOR35;G-protein coupled receptor 105;GPRv53;Pfi-013;SP9144
- Observed Band(KD):
- 45kD
- Background:
- Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by a family of histamine receptors, which are a subset of the G-protein coupled receptor superfamily. This gene encodes a histamine receptor that is predominantly expressed in haematopoietic cells. The protein is thought to play a role in inflammation and allergy reponses. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],
- Function:
- function:The H4 subclass of histamine receptors could mediate the histamine signals in peripheral tissues. Displays a significant level of constitutive activity (spontaneous activity in the absence of agonist).,induction:Expression is either up-regulated or down-regulated upon activation of the lymphoid tissues and this regulation may depend on the presence of IL-10 or IL-13.,miscellaneous:Does not bind diphenhydramine, loratadine, ranitidine, cimetidine and chlorpheniramine. Shows modest affinity for dimaprit, impromidine, clobenpropit, thioperamide, burimamide clozapine, immepip and imetit. The order of inhibitory activity was imetit > clobenpropit > burimamide > thioperamide. Clobenpropit behaves as a partial agonist, dimaprit and impromidine show some agonist activity while clozapine behaves as a full agonist. Thioperamide shows inverse agonism (enhances cAMP activity). The order of
- Subcellular Location:
- Cell membrane; Multi-pass membrane protein.
- Expression:
- Expressed primarily in the bone marrow and eosinophils. Shows preferential distribution in cells of immunological relevance such as T-cells, dendritic cells, monocytes, mast cells, neutrophils. Also expressed in a wide variety of peripheral tissues, including the heart, kidney, liver, lung, pancreas, skeletal muscle, prostate, small intestine, spleen, testis, colon, fetal liver and lymph node.
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- Antibody-FAQs
- Products Images
- Western Blot analysis of COLO cells using Histamine H4 Receptor Polyclonal Antibody
- Immunofluorescence analysis of LOVO cells, using HRH4 Antibody. The picture on the right is blocked with the synthesized peptide.