GPR135 Polyclonal Antibody
- Catalog No.:YT1970
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- GPR135
- Gene Name:
- GPR135
- Protein Name:
- Probable G-protein coupled receptor 135
- Human Gene Id:
- 64582
- Human Swiss Prot No:
- Q8IZ08
- Mouse Gene Id:
- 238252
- Mouse Swiss Prot No:
- Q7TQP2
- Rat Gene Id:
- 314213
- Rat Swiss Prot No:
- Q7TQN7
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human GPR135. AA range:371-420
- Specificity:
- GPR135 Polyclonal Antibody detects endogenous levels of GPR135 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- GPR135;Probable G-protein coupled receptor 135
- Observed Band(KD):
- 48kD
- Background:
- function:Orphan receptor.,similarity:Belongs to the G-protein coupled receptor 1 family.,
- Function:
- function:Orphan receptor.,similarity:Belongs to the G-protein coupled receptor 1 family.,
- Subcellular Location:
- Cell membrane ; Multi-pass membrane protein . Endosome membrane ; Multi-pass membrane protein . Colocalizes with ARRB2/beta-arrestin-2 in the endosome. .
- Expression:
- Testis,
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- May 20-2022
- Cell-Based ELISA│解您多样本WB检测之困扰
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Western blot analysis of lysates from NIH/3T3 cells, using GPR135 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).