GNL3L Polyclonal Antibody
- Catalog No.：YT1938
- Applications：WB;IHC;IF;ELISA
- Reactivity：Human;Rat;Mouse;Cat
- Target：
- GNL3L
- Fields：
- >>Ribosome biogenesis in eukaryotes
- Gene Name：
- GNL3L
- Protein Name：
- Guanine nucleotide-binding protein-like 3-like protein
- Human Gene Id：
- 54552
- Human Swiss Prot No：
- Q9NVN8
- Mouse Swiss Prot No：
- Q6PGG6
- Immunogen：
- The antiserum was produced against synthesized peptide derived from human GNL3L. AA range:71-120
- Specificity：
- GNL3L Polyclonal Antibody detects endogenous levels of GNL3L protein.
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Polyclonal, Rabbit,IgG
- Dilution：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
- Purification：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration：
- 1 mg/ml
- Storage Stability：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name：
- GNL3L;Guanine nucleotide-binding protein-like 3-like protein
- Observed Band(KD)：
- 66kD
- Background：
- The protein encoded by this gene appears to be a nucleolar GTPase that is essential for ribosomal pre-rRNA processing and cell proliferation. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, May 2010],
- Function：
- domain:In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.,function:Required for normal processing of ribosomal pre-rRNA. Required for cell proliferation. Binds GTP.,similarity:Belongs to the MMR1/HSR1 GTP-binding protein family.,
- Subcellular Location：
- Nucleus, nucleolus .
- Expression：
- Lung,Skin,
Profiling of Host Cell Response to Successive Canine Parvovirus Infection Based on Kinetic Proteomic Change Identification. Scientific Reports 2016 Jul 13 WB Cat F81 cell
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- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using GNL3L Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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- Western Blot analysis of HT29 cells using GNL3L Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Western Blot analysis of customer's (cat sample) using GNL3L Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Western blot analysis of mouse-brain HELA KB SH-SY5Y 293T 3T3 Mouse-kidney lysis using GNL3L antibody. Antibody was diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Western blot analysis of lysates from 293, HeLa, and HT-29 cells, using GNL3L Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HUVECcells using GNL3L antibody.
- Immunohistochemical analysis of paraffin-embedded human brain. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
