FAST-1/2 Polyclonal Antibody

    • Catalog No.:YT1679
    • Applications:WB;IF;ELISA
    • Reactivity:Human;Mouse
      • Target:
      • FAST-1/2
      • Gene Name:
      • FOXH1
      • Protein Name:
      • Forkhead box protein H1
      • Human Gene Id:
      • 8928
      • Human Swiss Prot No:
      • O75593
      • Mouse Swiss Prot No:
      • O88621
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human FOXH1. AA range:10-59
      • Specificity:
      • FAST-1/2 Polyclonal Antibody detects endogenous levels of FAST-1/2 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • FOXH1;FAST1;FAST2;Forkhead box protein H1;Forkhead activin signal transducer 1;Fast-1;hFAST-1;Forkhead activin signal transducer 2;Fast-2
      • Observed Band(KD):
      • 40kD
      • Background:
      • FOXH1 encodes a human homolog of Xenopus forkhead activin signal transducer-1. FOXH1 protein binds SMAD2 and activates an activin response element via binding the DNA motif TGT(G/T)(T/G)ATT. [provided by RefSeq, Jul 2008],
      • Function:
      • domain:The FM region is required for binding smad2/smad4 complexes. FM2 is more effective than FM1 and only interacts with phosphorylated smad2 that is in an activated smad complex.,function:Transcriptional activator. Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element).,similarity:Contains 1 fork-head DNA-binding domain.,subunit:Interacts with the MH2 domains of SMAD2 and SMAD3.,tissue specificity:Ubiquitous.,
      • Subcellular Location:
      • Nucleus.
      • Expression:
      • Ubiquitous.
      • Products Images
      • Western Blot analysis of various cells using FAST-1/2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
      • Immunofluorescence analysis of A549 cells, using FOXH1 Antibody. The picture on the right is blocked with the synthesized peptide.