• Target：
• DNA pol α

• Fields：
• >>DNA replication

• Gene Name：
• POLA1

• Protein Name：
• DNA polymerase alpha catalytic subunit

• Human Gene Id：
• 5422

• Human Swiss Prot No：
• P09884

• Mouse Swiss Prot No：
• P33609

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human DNA Polymerase alpha. AA range:81-130

• Specificity：
• DNA pol α Polyclonal Antibody detects endogenous levels of DNA pol α protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• POLA1;POLA;DNA polymerase alpha catalytic subunit;DNA polymerase alpha catalytic subunit p180

• Observed Band(KD)：
• 165kD

• Background：
• This gene encodes the catalytic subunit of DNA polymerase, which together with a regulatory and two primase subunits, forms the DNA polymerase alpha complex. The catalytic subunit plays an essential role in the initiation of DNA replication. [provided by RefSeq, Mar 2010],

• Function：
• catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,function:Polymerase alpha in a complex with DNA primase is a replicative polymerase.,miscellaneous:In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.,PTM:A 165 kDa form is probably produced by proteolytic cleavage at Lys-124.,similarity:Belongs to the DNA polymerase type-B family.,subunit:Interacts with SV40 Large T antigen; this interaction allows viral DNA replication.,

• Subcellular Location：
• Nucleus . Cytoplasm, cytosol . In the cytosol, colocalizes with RNA:DNA hybrids with a speckled pattern. .

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