CD130 Polyclonal Antibody
- Catalog No.:YT0721
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse
- Target:
- CD130/gp130
- Fields:
- >>Cytokine-cytokine receptor interaction;>>Viral protein interaction with cytokine and cytokine receptor;>>Signaling pathways regulating pluripotency of stem cells;>>JAK-STAT signaling pathway;>>Th17 cell differentiation;>>Kaposi sarcoma-associated herpesvirus infection;>>Coronavirus disease - COVID-19;>>Pathways in cancer;>>Viral carcinogenesis
- Gene Name:
- IL6ST
- Protein Name:
- Interleukin-6 receptor subunit beta
- Human Gene Id:
- 3572
- Human Swiss Prot No:
- P40189
- Mouse Gene Id:
- 16195
- Mouse Swiss Prot No:
- Q00560
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human CD130/gp130. AA range:748-797
- Specificity:
- CD130 Polyclonal Antibody detects endogenous levels of CD130 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- IL6ST;Interleukin-6 receptor subunit beta;IL-6 receptor subunit beta;IL-6R subunit beta;IL-6R-beta;IL-6RB;CDw130;Interleukin-6 signal transducer;Membrane glycoprotein 130;gp130;Oncostatin-M receptor subunit alpha;CD antigen CD130
- Observed Band(KD):
- 160kD
- Background:
- The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants have been described. A related pseudogene has been identified on chromosome 17. [provided by RefSeq, May 2014],
- Function:
- disease:Isoform 2 is an autoantigen found in rheumatoid arthritis (RA) but it is not specific to patients with RA.,domain:The box 1 motif is required for JAK interaction and/or activation.,domain:The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.,function:Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events.,induction:Leukemia inhibitory factor (LIF) and Oncostatin-M (OSM) activate the type I OSM receptor while only
- Subcellular Location:
- [Isoform 1]: Cell membrane ; Single-pass type I membrane protein .; [Isoform 2]: Secreted .
- Expression:
- Found in all the tissues and cell lines examined (PubMed:2261637). Expression not restricted to IL6 responsive cells (PubMed:2261637). ; [Isoform 2]: Expressed in blood serum (at protein level) (PubMed:24629561).
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- Antibody-FAQs
- Products Images
- Western Blot analysis of 293T COLO cells using CD130 Polyclonal Antibody diluted at 1:500
- Immunofluorescence analysis of NIH/3T3 cells, using CD130/gp130 Antibody. The picture on the right is blocked with the synthesized peptide.
- Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CD130/gp130 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from Jurkat cells, using CD130/gp130 Antibody. The lane on the right is blocked with the synthesized peptide.