• Target：
• ATP-citrate synthase

• Fields：
• >>Citrate cycle (TCA cycle);>>Metabolic pathways

• Gene Name：
• ACLY

• Protein Name：
• ATP-citrate synthase

• Human Gene Id：
• 47

• Human Swiss Prot No：
• P53396

• Mouse Gene Id：
• 104112

• Mouse Swiss Prot No：
• Q91V92

• Rat Gene Id：
• 24159

• Rat Swiss Prot No：
• P16638

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human ATP-Citrate Lyase. AA range:420-469

• Specificity：
• ATP-citrate synthase Polyclonal Antibody detects endogenous levels of ATP-citrate synthase protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• ACLY;ATP-citrate synthase;ATP-citrate;pro-S-)-lyase;ACL;Citrate cleavage enzyme

• Observed Band(KD)：
• 120kD

• Background：
• ATP citrate lyase(ACLY) Homo sapiens ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014],

• Function：
• catalytic activity:ADP + phosphate + acetyl-CoA + oxaloacetate = ATP + citrate + CoA.,function:ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.,similarity:In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.,similarity:In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.,subunit:Homotetramer.,

• Subcellular Location：
• Cytoplasm, cytosol .

• Expression：
• Brain,Epithelium,Hippocampus,Liver,Lymph,Platelet,

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