ATF-7 Polyclonal Antibody

    • Catalog No.:YT0390
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse
      • Target:
      • ATF-7
      • Gene Name:
      • ATF7
      • Protein Name:
      • Cyclic AMP-dependent transcription factor ATF-7
      • Human Swiss Prot No:
      • P17544
      • Mouse Swiss Prot No:
      • Q8R0S1
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human ATF7. AA range:41-90
      • Specificity:
      • ATF-7 Polyclonal Antibody detects endogenous levels of ATF-7 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • ATF7;ATFA;Cyclic AMP-dependent transcription factor ATF-7;cAMP-dependent transcription factor ATF-7;Activating transcription factor 7;Transcription factor ATF-A
      • Observed Band(KD):
      • 52kD
      • Background:
      • function:Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters.,similarity:Belongs to the bZIP family.,similarity:Contains 1 bZIP domain.,similarity:Contains 1 C2H2-type zinc finger.,subunit:Binds DNA as a dimer.,
      • Function:
      • function:Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters.,similarity:Belongs to the bZIP family.,similarity:Contains 1 bZIP domain.,similarity:Contains 1 C2H2-type zinc finger.,subunit:Binds DNA as a dimer.,
      • Subcellular Location:
      • Nucleus . Nucleus, nucleoplasm . Chromosome, telomere . Mainly nucleoplasmic. Restricted distribution to the perinuculear region. The sumoylated form locates to the nuclear periphery.; [Isoform 5]: Cytoplasm .
      • Expression:
      • Expressed in various tissues including heart, brain, placenta, lung and skeletal muscle. Highest levels in skeletal muscle. Lowest in lung and placenta. ; [Isoform 4]: Strongly expressed in skeletal muscle. Also expressed at lower levels in heart and lung.
      • Products Images
      • Western Blot analysis of various cells using ATF-7 Polyclonal Antibody
      • Western blot analysis of lysates from HeLa and HUVEC cells, using ATF7 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Immunohistochemical analysis of paraffin-embedded human Small intestinal stromal tumor. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).