• Target：
• ATF-3

• Gene Name：
• ATF3

• Protein Name：
• Cyclic AMP-dependent transcription factor ATF-3

• Human Gene Id：
• 467

• Human Swiss Prot No：
• P18847

• Mouse Gene Id：
• 11910

• Mouse Swiss Prot No：
• Q60765

• Rat Gene Id：
• 25389

• Rat Swiss Prot No：
• P29596

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human ATF3. AA range:131-180

• Specificity：
• ATF-3 Polyclonal Antibody detects endogenous levels of ATF-3 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500-2000;IHC 1:100-500;IF ICC 1:100-500;ELISA 1:5000-20000

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• ATF3;Cyclic AMP-dependent transcription factor ATF-3;cAMP-dependent transcription factor ATF-3;Activating transcription factor 3

• Observed Band(KD)：
• 21kD

• Background：
• activating transcription factor 3(ATF3) Homo sapiens This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011],

• Function：
• function:This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.,similarity:Belongs to the bZIP family.,similarity:Belongs to the bZIP family. ATF subfamily.,similarity:Contains 1 bZIP domain.,subunit:Binds DNA as a homodimer or a heterodimer.,

• Subcellular Location：
• Nucleus .

• Expression：
• Amygdala,Brain,Cerebellum,Muscle,

Citrus alkaline extracts prevent endoplasmic reticulum stress in type II alveolar epithelial cells to ameliorate pulmonary fibrosis via the ATF3/PINK1 pathway. PHYTOMEDICINE Phytomedicine. 2021 Aug;89:153599 WB，IF Human 1 : 200，1:1000 Lung A549 cell

Wang, Zhichao, et al. "Citrus alkaline extract prevents endoplasmic reticulum stress in type II alveolar epithelial cells to ameliorate pulmonary fibrosis via the ATF3/PINK1 pathway." Phytomedicine (2021): 153599.

Development and Validation of Robust Ferroptosis-Related Genes in Myocardial Ischemia-Reperfusion Injury. Journal of Cardiovascular Development and Disease Yu Liu IHC Mouse 1:250 hearts

Amphiregulin secreted by umbilical cord Multipotent Stromal Cells protects against ferroptosis of macrophages via the Activating Transcription Factor 3-CD36 axis to alleviate endometrial fibrosis STEM CELLS Wang Jiali WB Mouse Raw264.7 cell

Platelet-rich plasma alleviates neuropathic pain in osteoarthritis by downregulating microglial activation BMC MUSCULOSKELETAL DISORDERS Yan Xiao IHC Rat 1:100 dorsal root ganglia (DRG) tissue

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