SGK3 (Phospho Thr320) rabbit pAb
- Catalog No.:YP1486
- Applications:WB;IHC
- Reactivity:Human;Rat;Mouse;
- Target:
- SGK3
- Fields:
- >>FoxO signaling pathway;>>PI3K-Akt signaling pathway
- Gene Name:
- SGK3 CISK SGKL
- Protein Name:
- SGK3 (Thr320)
- Human Gene Id:
- 100533105
- Human Swiss Prot No:
- Q96BR1
- Mouse Gene Id:
- 170755
- Mouse Swiss Prot No:
- Q9ERE3
- Rat Swiss Prot No:
- Q8R4V0
- Immunogen:
- Synthesized phosho peptide around human SGK3 (Thr320)
- Specificity:
- This antibody detects endogenous levels of Human SGK3 (phospho-Thr320)
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500-2000;IHC 1:50-300
- Purification:
- The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- Serine/threonine-protein kinase Sgk3 (EC 2.7.11.1) (Cytokine-independent survival kinase) (Serum/glucocorticoid-regulated kinase 3) (Serum/glucocorticoid-regulated kinase-like)
- Observed Band(KD):
- 54kD
- Background:
- This gene is a member of the Ser/Thr protein kinase family and encodes a phosphoprotein with a PX (phox homology) domain. The protein phosphorylates several target proteins and has a role in neutral amino acid transport and activation of potassium and chloride channels. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008],
- Function:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Two specific sites, one in the kinase domain (Thr-320) and the other in the C-terminal regulatory region (Ser-486), need to be phosphorylated for its full activation.,function:Involved in the activation of potassium channels. Mediates cell IL-3-dependent survival signals.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 PX (phox homology) domain.,subcellular location:Localized in vesicle-like structures and in the early endosome.,tissue specificity:Expressed in most tissues with highest levels in pancreas, kidney liver, heart and brain and lower levels in lung, placenta and skeletal muscle.,
- Subcellular Location:
- Cytoplasmic vesicle . Early endosome. Recycling endosome. Endosomal localization is a prerequisite for complete kinase activity. It is essential for its colocalization with the kinase responsible for phosphorylating Ser-486 thus allowing PDPK1 phosphorylation of Thr-320 resulting in complete activation of SGK3. Localized in vesicle-like structures and in the early endosome. Colocalizes with SLC9A3/NHE3 in the recycling endosomes.
- Expression:
- Expressed in most tissues with highest levels in pancreas, kidney liver, heart and brain and lower levels in lung, placenta and skeletal muscle. Expression is higher in ER-positive breast tumors than ER-negative breast tumors.
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- Antibody-FAQs
- Products Images
- Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).