• Target：
• Adducin α

• Gene Name：
• ADD1

• Protein Name：
• Alpha-adducin

• Human Gene Id：
• 118

• Human Swiss Prot No：
• P35611

• Mouse Gene Id：
• 11518

• Mouse Swiss Prot No：
• Q9QYC0

• Rat Gene Id：
• 24170

• Rat Swiss Prot No：
• Q63028

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human ADD1 around the phosphorylation site of Thr445. AA range:411-460

• Specificity：
• Phospho-Adducin α (T445) Polyclonal Antibody detects endogenous levels of Adducin α protein only when phosphorylated at T445.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• ADD1;ADDA;Alpha-adducin;Erythrocyte adducin subunit alpha

• Molecular Weight(Da)：
• 81kD

• Background：
• adducin 1(ADD1) Homo sapiens Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq, Jul 2008],

• Function：
• alternative products:Additional isoforms seem to exist,domain:Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.,function:Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.,PTM:The N-terminus is blocked.,similarity:Belongs to the aldolase class II family. Adducin subfamily.,subunit:Heterodimer of an alpha and a beta subunit or an alpha and a gamma subunit. Binds ROCK1.,tissue specificity:Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.,

• Subcellular Location：
• Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side.

• Expression：
• Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.

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