• Target：
• Histone H3

• Fields：
• >>Neutrophil extracellular trap formation;>>Alcoholism;>>Shigellosis;>>Transcriptional misregulation in cancer;>>Systemic lupus erythematosus

• Gene Name：
• HIST1H3A

• Protein Name：
• Histone H3.1

• Human Gene Id：
• 8350/8351/8352/8353/8354/8355/8356/8357/8358/8968

• Human Swiss Prot No：
• P68431/Q71DI3/P84243

• Mouse Gene Id：
• 319152/15077/15078

• Rat Gene Id：
• 291159/100361558

• Rat Swiss Prot No：
• Q6LED0/P84245

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human Histone H3.1 around the phosphorylation site of Ser10. AA range:1-50

• Specificity：
• Phospho-Histone H3 (S10) Polyclonal Antibody detects endogenous levels of Histone H3 protein only when phosphorylated at S10.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ICC:IF 1:200 - 1:1000. ELISA: 1:10000. IHC-f,Flow Cyt 1:500

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• HIST1H3A;H3FA;HIST1H3B;H3FL;HIST1H3C;H3FC;HIST1H3D;H3FB;HIST1H3E;H3FD;HIST1H3F;H3FI;HIST1H3G;H3FH;HIST1H3H;H3FK;HIST1H3I;H3FF;HIST1H3J;H3FJ;Histone H3.1;Histone H3/a;Histone H3/b;Histone H3/c;Histone H3/d;Histone H3

• Observed Band(KD)：
• 15kD

• Background：
• Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],

• Function：
• caution:Was originally (PubMed:2587222) thought to originate from mouse.,developmental stage:Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.,function:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.,mass spectrometry:Monoisotopic with N-acetylserine PubMed:16457589,miscellaneous:This histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2).,PTM:Acetylation is generally l

• Subcellular Location：
• Nucleus. Chromosome.

• Expression：
• Blood,Epithelium,Kidney,Lung,Ovary,Spleen,Uterus,

VhaAC39-1 regulates gut homeostasis and affects the health span in Drosophila. MECHANISMS OF AGEING AND DEVELOPMENT2022 Jun;204:111673. Drosophila 1:200 intestinal stem cells (ISCs)

Plumbagin inhibits the proliferation and survival of esophageal cancer cells by blocking STAT3-PLK1-AKT signaling. Cell Death & Disease Cell Death Dis. 2018 Jan;9(2):1-13 WB Human KYSE150 cell, KYSE450 cell

Splicing factor Srsf5 deletion disrupts alternative splicing and causes noncompaction of ventricular myocardium. iScience Iscience. 2021 Oct;24:103097 IHC Mouse 1:500 Heart

Elevated TAB182 enhances the radioresistance of esophageal squamous cell carcinoma through G2-M checkpoint modulation. Cancer Medicine Cancer Med-Us. 2021 May;10(9):3101-3112 FC Human TE-1 cell， TE-10 cell

Hypoxic culture enhances the expansion of rat bone marrow-derived mesenchymal stem cells via the regulatory pathways of cell division and apoptosis. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL 2018 Aug 22 IHC Rat rBMSCs

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs