Histone H3 (phospho Ser10) Polyclonal Antibody

    • Catalog No.:YP0129
    • Applications:WB,IHC-p,IHC-f,Flow Cyt,ICC,IF,ELISA
    • Reactivity:Human,Mouse,Rat,Drosophila melanogaster
      • Gene Name:
      • HIST1H3A
      • Protein Name:
      • Histone H3.1
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human Histone H3.1 around the phosphorylation site of Ser10. AA range:1-50
      • Specificity:
      • Phospho-Histone H3 (S10) Polyclonal Antibody detects endogenous levels of Histone H3 protein only when phosphorylated at S10.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. IHC: 1/100 - 1/300. ICC/IF: 1/200 - 1/1000. ELISA: 1/10000. IHC-f,Flow Cyt 1:500
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b
      • MolecularWeight(Da):
      • 15273
      • Observed Band(KD):
      • 15
      • Background:
      • histone cluster 1 H3 family member a(HIST1H3A) Homo sapiens Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],
      • Function:
      • caution:Was originally (PubMed:2587222) thought to originate from mouse.,developmental stage:Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.,function:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.,mass spectrometry:Monoisotopic with N-acetylserine PubMed:16457589,miscellaneous:This histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2).,PTM:Acetylation is generally l
      • Subcellular Location:
      • nuclear chromosome,nuclear chromosome, telomeric region,nucleosome,nuclear nucleosome,extracellular region,nucleus,nucleoplasm,cell-cell adherens junction,membrane,protein complex,extracellular exosome,
      • Expression:
      • Blood,Epithelium,Kidney,Lung,Ovary,Spleen,Uterus,

      Zhang, Jun, et al. "Hypoxic culture enhances the expansion of rat bone marrow-derived mesenchymal stem cells via the regulatory pathways of cell division and apoptosis." In Vitro Cellular & Developmental Biology-Animal 54.9 (2018): 666-676.
      Catalog No.:YP0129

      • Products Images
      • Western Blot analysis of various cells using Phospho-Histone H3 (S10) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
      • Western Blot analysis of KB cells using Phospho-Histone H3 (S10) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Histone H3.1 (Phospho-Ser10) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from NIH/3T3 cells and HepG2 cells, using Histone H3.1 (Phospho-Ser10) Antibody. The lane on the right is blocked with the phospho peptide.