SPRE2 rabbit pAb
- Catalog No.:YT6816
- Applications:WB;IHC
- Reactivity:Human;Mouse;Rat
- Target:
- SPRE2
- Gene Name:
- SPRED2
- Protein Name:
- SPRE2
- Human Gene Id:
- 200734
- Human Swiss Prot No:
- Q7Z698
- Mouse Gene Id:
- 114716
- Mouse Swiss Prot No:
- Q924S7
- Rat Gene Id:
- 305539
- Rat Swiss Prot No:
- Q3C2P8
- Immunogen:
- Synthesized peptide derived from human SPRE2 AA range: 68-118
- Specificity:
- This antibody detects endogenous levels of SPRE2 at Human/Mouse/Rat
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500-2000;IHC 1:50-300
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Molecular Weight(Da):
- 46kD
- Background:
- SPRED2 is a member of the Sprouty (see SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see MAPK1; MIM 176948) (Nonami et al., 2004 [PubMed 15465815]).[supplied by OMIM, Mar 2008],
- Function:
- function:Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase.,PTM:Phosphorylated on tyrosine. Phosphorylation of Tyr-228 and Tyr-231 are required for ubiquitination.,PTM:Ubiquitinated; leading to degradation by the proteasome.,similarity:Contains 1 KBD domain.,similarity:Contains 1 SPR (sprouty) domain.,similarity:Contains 1 WH1 domain.,subcellular location:Especially found in the cytoplasm of the stratum spinosum cells called membrane-coated granules.,subunit:Interacts with Ras (By similarity). Homodimer and heterodimer. Able to interact with SPRED1 to form heterodimers.,tissue specificity:Expressed in liver, skin, small intestine, salivary gland and prostate.,
- Subcellular Location:
- Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasmic vesicle, secretory vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm . Detected in the cytoplasm of the stratum spinosum cells, where it is associated with cytoplasmic vesicles that are supposed to be secretory granules. .
- Expression:
- Expressed in liver, skin, small intestine, salivary gland and prostate.
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- Antibody-FAQs
- Products Images
- Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°over night
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).