• Target：
• CD166

• Fields：
• >>Cell adhesion molecules

• Gene Name：
• ALCAM

• Protein Name：
• CD166 antigen

• Human Gene Id：
• 214

• Human Swiss Prot No：
• Q13740

• Mouse Gene Id：
• 11658

• Mouse Swiss Prot No：
• Q61490

• Rat Gene Id：
• 79559

• Rat Swiss Prot No：
• O35112

• Immunogen：
• The antiserum was produced against synthesized peptide derived from the C-terminal region of human ALCAM. AA range:481-530

• Specificity：
• CD166 Polyclonal Antibody detects endogenous levels of CD166 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IF 1:50-200 WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• ALCAM;MEMD;CD166 antigen;Activated leukocyte cell adhesion molecule;CD166

• Observed Band(KD)：
• 65kD

• Background：
• This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011],

• Function：
• domain:The CD6 binding site is located in the N-terminal Ig-like domain.,function:Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system.,similarity:Contains 2 Ig-like V-type (immunoglobulin-like) domains.,similarity:Contains 3 Ig-like C2-type (immunoglobulin-like) domains.,tissue specificity:Spleen, placenta, liver, and weakly in liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Preferentially expressed in highly metastasizing melanoma cell lines.,

• Subcellular Location：
• Cell membrane ; Single-pass type I membrane protein . Cell projection, axon . Cell projection, dendrite . Detected at the immunological synapse, i.e, at the contact zone between antigen-presenting dendritic cells and T-cells (PubMed:15294938, PubMed:16352806). Colocalizes with CD6 and the TCR/CD3 complex at the immunological synapse (PubMed:15294938). .; [Isoform 3]: Secreted .

• Expression：
• Detected on hematopoietic stem cells derived from umbilical cord blood (PubMed:24740813). Detected on lymph vessel endothelial cells, skin and tonsil (PubMed:23169771). Detected on peripheral blood monocytes (PubMed:15048703). Detected on monocyte-derived dendritic cells (at protein level) (PubMed:16352806). Detected at low levels in spleen, placenta, liver (PubMed:9502422). Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells (PubMed:7760007). Isoform 1 and isoform 3 are detected in vein and artery endothelial cells, astrocytes, keratinocytes and artery smooth muscle cells (PubMed:15496415). Expressed by neurons in the brain. Restricted expression in tumor cell lines. Detected in highly metastasizing melanoma cell lines (PubMed:9502422).

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