N4BP1 Polyclonal Antibody

    • Catalog No.:YT2962
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse
      • Target:
      • N4BP1
      • Gene Name:
      • N4BP1
      • Protein Name:
      • NEDD4-binding protein 1
      • Human Gene Id:
      • 9683
      • Human Swiss Prot No:
      • O75113
      • Mouse Swiss Prot No:
      • Q6A037
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human N4BP1. AA range:383-432
      • Specificity:
      • N4BP1 Polyclonal Antibody detects endogenous levels of N4BP1 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • N4BP1;KIAA0615;NEDD4-binding protein 1;N4BP1
      • Observed Band(KD):
      • 100kD
      • Background:
      • PTM:Monoubiquitinated.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the N4BP1 family.,subcellular location:In discrete circular structures.,subunit:Binds NEDD4.,tissue specificity:Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary.,
      • Function:
      • PTM:Monoubiquitinated.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the N4BP1 family.,subcellular location:In discrete circular structures.,subunit:Binds NEDD4.,tissue specificity:Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary.,
      • Subcellular Location:
      • Cytoplasm, cytosol . Nucleus . Nucleus, nucleolus . Nucleus, PML body . Primarily localizes to the nucleolus. Also localizes to the PML nuclear bodies, when desumoylated. .
      • Expression:
      • Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary.
      • Products Images
      • Western Blot analysis of various cells using N4BP1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
      • Western Blot analysis of K562 cells using N4BP1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
      • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
      • Western blot analysis of lysates from MCF-7 and Jurkat/293 cells, using N4BP1 Antibody. The lane on the right is blocked with the synthesized peptide.