• Target：
• PHF8

• Gene Name：
• PHF8 KIAA1111 ZNF422

• Protein Name：
• Histone lysine demethylase PHF8 (EC 1.14.11.27) (PHD finger protein 8)

• Human Gene Id：
• 23133

• Human Swiss Prot No：
• Q9UPP1

• Mouse Gene Id：
• 320595

• Mouse Swiss Prot No：
• Q80TJ7

• Immunogen：
• Synthesized peptide derived from human C-ternal PHF8

• Specificity：
• This antibody detects endogenous levels of PHF8 at Human, Mouse

• Formulation：
• Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500-2000 IHC 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• Histone lysine demethylase PHF8 (EC 1.14.11.27) (PHD finger protein 8)

• Molecular Weight(Da)：
• 117kD

• Function：
• Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes. Required for brain development, probably by regulating expression of neuron-specific genes. Only has activity toward H4K20Me1 when nucleosome is used as a substrate and when not histone octamer is used as substrate. May also have weak activity

• Subcellular Location：
• Nucleus . Nucleus, nucleolus . Recruited to H3K4me3 sites on chromatin during interphase (PubMed:20622854). Dissociates from chromatin when cells enter mitosis (PubMed:20622854). .

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