RN139 rabbit pAb
- Catalog No.:YT7653
- Applications:WB;IHC
- Reactivity:Human;Mouse
- Target:
- RN139
- Gene Name:
- RNF139 TRC8
- Protein Name:
- RN139
- Human Gene Id:
- 11236
- Human Swiss Prot No:
- Q8WU17
- Mouse Gene Id:
- 75841
- Mouse Swiss Prot No:
- Q7TMV1
- Immunogen:
- Synthesized peptide derived from human RN139 AA range: 10-60
- Specificity:
- This antibody detects endogenous levels of RN139 at Human/Mouse
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500-2000;IHC 1:50-300
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Molecular Weight(Da):
- 73kD
- Background:
- The protein encoded by this gene is a multi-membrane spanning protein containing a RING-H2 finger. This protein is located in the endoplasmic reticulum, and has been shown to possess ubiquitin ligase activity. This gene was found to be interrupted by a t(3:8) translocation in a family with hereditary renal and non-medulary thyroid cancer. Studies of the Drosophila counterpart suggested that this protein may interact with tumor suppressor protein VHL, as well as with COPS5/JAB1, a protein responsible for the degradation of tumor suppressor CDKN1B/P27KIP. [provided by RefSeq, Jul 2008],
- Function:
- disease:Defects in RNF139 are a cause of renal cell carcinoma (RCC) [MIM:144700]. A chromosomal aberration involving RNF139 is found in hereditary RCC. Translocation (3;8)(q14.2;q24.1) with FHIT. The result is RNF139 is found to be fused to FHIT and disrupted within the sterol-sensing domain. In contrast, the FHIT coding region is maintained and expressed. Sporadic RCC, where an acquired mutation in RNF139 results in the duplication of 12 nucleotides in the 5'-UTR, has also been identified.,domain:The RING-type zinc finger domain may be essential for ubiquitin ligase activity.,function:Potential tumor suppressor for renal cell carcinoma. Plays a role in mediating ubiquitination. May function as a signaling receptor.,similarity:Contains 1 RING-type zinc finger.,subunit:Interacts with VHL.,tissue specificity:Highly expressed in testis, placenta and adrenal gland. Moderate expression in hea
- Subcellular Location:
- Endoplasmic reticulum membrane ; Multi-pass membrane protein .
- Expression:
- Highly expressed in testis, placenta and adrenal gland. Moderate expression in heart, brain, liver, skeletal muscle and pancreas, and low expression in lung and kidney.
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- Antibody-FAQs
- Products Images
- Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°over night
- Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).