PIGM rabbit pAb
- Catalog No.:YT7624
- Applications:WB;IHC
- Reactivity:Human;Mouse;Rat
- Target:
- PIGM
- Fields:
- >>Glycosylphosphatidylinositol (GPI)-anchor biosynthesis;>>Metabolic pathways
- Gene Name:
- PIGM
- Protein Name:
- PIGM
- Human Gene Id:
- 93183
- Human Swiss Prot No:
- Q9H3S5
- Mouse Gene Id:
- 67556
- Mouse Swiss Prot No:
- Q8C2R7
- Rat Gene Id:
- 79112
- Rat Swiss Prot No:
- Q9EQY6
- Immunogen:
- Synthesized peptide derived from human PIGM AA range: 147-197
- Specificity:
- This antibody detects endogenous levels of PIGM at Human/Mouse/Rat
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500-2000;IHC 1:50-300
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Molecular Weight(Da):
- 47kD
- Background:
- This gene encodes a transmembrane protein that is located in the endoplasmic reticulum and is involved in GPI-anchor biosynthesis. The glycosylphosphatidylinositol (GPI)-anchor is a glycolipid which contains three mannose molecules in its core backbone. The GPI-anchor is found on many blood cells and serves to anchor proteins to the cell surface. This gene encodes a mannosyltransferase, GPI-MT-I, that transfers the first mannose to GPI on the lumenal side of the endoplasmic reticulum. [provided by RefSeq, Jul 2008],
- Function:
- disease:Defects in PIGM are the cause of glycosylphosphatidylinositol deficiency (GPID) [MIM:610293]. GPID is an autosomal recessive trait that results in a propensity to venous thrombosis and seizures. Deficiency is due to a point mutation in the regulatory sequences of PIGM that disrupts binding of the transcription factor SP1 to its cognate promoter motif, leading to a strong reduction of expression.,function:Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the first alpha-1,4-mannose to GlcN-acyl-PI during GPI precursor assembly.,pathway:Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.,similarity:Belongs to the PIGM family.,
- Subcellular Location:
- Endoplasmic reticulum membrane ; Multi-pass membrane protein .
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- Antibody-FAQs
- Products Images
- Western blot analysis of lysates from A549 cells, primary antibody was diluted at 1:1000, 4°over night
- Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).