DAD1 rabbit pAb
- Catalog No.:YT7600
- Applications:WB
- Reactivity:Human;Mouse;Rat
- Target:
- DAD1
- Fields:
- >>N-Glycan biosynthesis;>>Various types of N-glycan biosynthesis;>>Metabolic pathways;>>Protein processing in endoplasmic reticulum
- Gene Name:
- DAD1
- Protein Name:
- DAD1
- Human Gene Id:
- 1603
- Human Swiss Prot No:
- P61803
- Mouse Gene Id:
- 13135
- Mouse Swiss Prot No:
- P61804
- Rat Gene Id:
- 192275
- Rat Swiss Prot No:
- P61805
- Immunogen:
- Synthesized peptide derived from human DAD1 AA range: 38-88
- Specificity:
- This antibody detects endogenous levels of DAD1 at Human/Mouse/Rat
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500-2000
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Molecular Weight(Da):
- 12kD
- Background:
- DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes. [provided by RefSeq, Jul 2008],
- Function:
- catalytic activity:Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.,function:Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis.,similarity:Belongs to the DAD/OST2 family.,subunit:Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2,
- Subcellular Location:
- Endoplasmic reticulum membrane; Multi-pass membrane protein .
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- Antibody-FAQs
- Products Images
- Western blot analysis of lysates from MDA-MB cells, primary antibody was diluted at 1:1000, 4°over night