RIFK rabbit pAb

    • Catalog No.:YT7290
    • Applications:WB;ELISA;IHC
    • Reactivity:Human;Mouse
      • Target:
      • RIFK
      • Fields:
      • >>Riboflavin metabolism;>>Metabolic pathways;>>Biosynthesis of cofactors
      • Gene Name:
      • RFK
      • Protein Name:
      • RIFK
      • Human Swiss Prot No:
      • Q969G6
      • Mouse Swiss Prot No:
      • Q8CFV9
      • Immunogen:
      • Synthesized peptide derived from human RIFK AA range: 82-132
      • Specificity:
      • This antibody detects endogenous levels of RIFK at Human/Mouse
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Molecular Weight(Da):
      • 17kD
      • Background:
      • Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]).[supplied by OMIM, Nov 2009],
      • Function:
      • catalytic activity:ATP + riboflavin = ADP + FMN.,cofactor:Zinc or magnesium.,function:Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN).,pathway:Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.,subunit:Monomer.,tissue specificity:Detected in brain, placenta and urinary bladder.,
      • Subcellular Location:
      • Cytoplasm .
      • Expression:
      • Detected in brain, placenta and urinary bladder.
      • Products Images
      • Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night
      • Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).