RIFK rabbit pAb
- Catalog No.:YT7290
- Applications:WB;ELISA;IHC
- Reactivity:Human;Mouse
- Target:
- RIFK
- Fields:
- >>Riboflavin metabolism;>>Metabolic pathways;>>Biosynthesis of cofactors
- Gene Name:
- RFK
- Protein Name:
- RIFK
- Human Gene Id:
- 55312
- Human Swiss Prot No:
- Q969G6
- Mouse Gene Id:
- 54391
- Mouse Swiss Prot No:
- Q8CFV9
- Immunogen:
- Synthesized peptide derived from human RIFK AA range: 82-132
- Specificity:
- This antibody detects endogenous levels of RIFK at Human/Mouse
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Molecular Weight(Da):
- 17kD
- Background:
- Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]).[supplied by OMIM, Nov 2009],
- Function:
- catalytic activity:ATP + riboflavin = ADP + FMN.,cofactor:Zinc or magnesium.,function:Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN).,pathway:Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.,subunit:Monomer.,tissue specificity:Detected in brain, placenta and urinary bladder.,
- Subcellular Location:
- Cytoplasm .
- Expression:
- Detected in brain, placenta and urinary bladder.
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- Antibody-FAQs
- Products Images
- Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night
- Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).