• Target：
• SIN1

• Fields：
• >>mTOR signaling pathway

• Gene Name：
• MAPKAP1 MIP1 SIN1

• Protein Name：
• SIN1

• Human Gene Id：
• 79109

• Human Swiss Prot No：
• Q9BPZ7

• Mouse Gene Id：
• 227743

• Mouse Swiss Prot No：
• Q8BKH7

• Rat Gene Id：
• 296648

• Rat Swiss Prot No：
• Q6AYF1

• Immunogen：
• Synthesized peptide derived from human SIN1 AA range: 193-243

• Specificity：
• This antibody detects endogenous levels of SIN1 at Human/Mouse/Rat

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Molecular Weight(Da)：
• 57kD

• Background：
• This gene encodes a protein that is highly similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. [provided by RefSeq, Jul 2008],

• Function：
• function:Essential component of the TORC2 complex, which plays a critical role in AKT1 'Ser-473' phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization. Inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAs signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription.,similarity:Belongs to the SIN1 family.,subunit:All isoforms except isoform 4 are able to be part of the target of rapamycin 2 complex (TORC2) comprised of FRAP1, LST8, PROTOR1, RICTOR and MAPKAP1. Interacts with ATF2, MAP3K2 and MAPK8. Interacts with GTP-bound HRAS and KRAS. Interacts with IFNAR2.,tissue specificity:Ubiquitously expressed, with highest levels in heart and skeletal muscle.,

• Subcellular Location：
• Cell membrane; Peripheral membrane protein. Cytoplasmic vesicle. Nucleus.

• Expression：
• Ubiquitously expressed, with highest levels in heart and skeletal muscle.

SP1-Induced Upregulation of LncRNA AFAP1-AS1 Promotes Tumor Progression in Triple-Negative Breast Cancer by Regulating mTOR Pathway. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Yidong Zhou WB Human 1:500 MDA-231 cell,MDA-MB-468 cell

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