DHE3 rabbit pAb
- Catalog No.:YT7087
- Applications:WB
- Reactivity:Human;Mouse;Rat
- Target:
- DHE3
- Fields:
- >>Arginine biosynthesis;>>Alanine, aspartate and glutamate metabolism;>>Nitrogen metabolism;>>Metabolic pathways;>>Carbon metabolism;>>Necroptosis;>>Proximal tubule bicarbonate reclamation
- Gene Name:
- GLUD1 GLUD
- Protein Name:
- DHE3
- Human Gene Id:
- 2746
- Human Swiss Prot No:
- P00367
- Mouse Gene Id:
- 14661
- Mouse Swiss Prot No:
- P26443
- Rat Gene Id:
- 24399
- Rat Swiss Prot No:
- P10860
- Immunogen:
- Synthesized peptide derived from human DHE3
- Specificity:
- This antibody detects endogenous levels of DHE3 at Human/Mouse/Rat
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500-2000
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Molecular Weight(Da):
- 61kD
- Background:
- This gene encodes glutamate dehydrogenase, which is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X. [provided by RefSeq, Jan 2016],
- Function:
- catalytic activity:L-glutamate + H(2)O + NAD(P)(+) = 2-oxoglutarate + NH(3) + NAD(P)H.,disease:Defects in GLUD1 are the cause of hyperinsulinism-hyperammonemia syndrome (HHS) [MIM:606762]. Elevated oxidation rate of glutamate to alpha-ketoglutarate stimulates insulin secretion in the pancreatic beta cells, while they impair detoxification of ammonium in the liver.,enzyme regulation:Subject to allosteric regulation. Activated by ADP. Inhibited by GTP and ATP. ADP can occupy the NADH binding site and activate the enzyme.,function:May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.,online information:Glutamate dehydrogenase 1 entry,similarity:Belongs to the Glu/Leu/Phe/Val dehydrogenases family.,subunit:Homohexamer.,
- Subcellular Location:
- Mitochondrion . Endoplasmic reticulum . Mostly translocates into the mitochondria, only a small amount of the protein localizes to the endoplasmic reticulum. .
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- Antibody-FAQs
- Products Images
- Western blot analysis of lysates from MDA-MB cells, primary antibody was diluted at 1:1000, 4°over night