SHPRH rabbit pAb
- Catalog No.:YT6957
- Applications:WB;IF;ELISA;IHC
- Reactivity:Human;Mouse
- Target:
- SHPRH
- Gene Name:
- SHPRH KIAA2023
- Protein Name:
- SHPRH
- Human Gene Id:
- 257218
- Human Swiss Prot No:
- Q149N8
- Mouse Gene Id:
- 268281
- Mouse Swiss Prot No:
- Q7TPQ3
- Immunogen:
- Synthesized peptide derived from human SHPRH AA range: 9-59
- Specificity:
- This antibody detects endogenous levels of SHPRH at Human/Mouse
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500-2000; IF ICC 1:50-200;ELISA 1:2000-20000;IHC 1:50-200
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Molecular Weight(Da):
- 185kD
- Background:
- SHPRH is a ubiquitously expressed protein that contains motifs characteristics of several DNA repair proteins, transcription factors, and helicases. SHPRH is a functional homolog of S. cerevisiae RAD5 (Unk et al., 2006 [PubMed 17108083]).[supplied by OMIM, Mar 2008],
- Function:
- domain:The RING finger mediates E3 ubiquitin ligase activity.,function:E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'.,pathway:Protein modification; protein ubiquitination.,similarity:Belongs to the SNF2/RAD54 helicase family.,similarity:Contains 1 helicase ATP-binding domain.,similarity:Contains 1 helicase C-terminal domain.,similarity:Contains 1 histone H1 domain.,similarity:Contains 1 PHD-type zinc finger.,similarity:Contains 1 RING-type zinc finger.,subunit:Homodimer. Interacts with PCNA, UBE2N and RAD18.,tissue specificity:Broadly expressed.,
- Subcellular Location:
- nucleosome,nucleus,
- Expression:
- Broadly expressed.
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- Antibody-FAQs
- Products Images
- Western blot analysis of lysates from MDA-MB cells, primary antibody was diluted at 1:1000, 4°over night
- Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).