UBA3 rabbit pAb
- Catalog No.:YT6896
- Applications:WB
- Reactivity:Human;Mouse;Rat
- Target:
- UBA3
- Fields:
- >>Ubiquitin mediated proteolysis
- Gene Name:
- UBA3 UBE1C
- Protein Name:
- UBA3
- Human Gene Id:
- 9039
- Human Swiss Prot No:
- Q8TBC4
- Mouse Gene Id:
- 22200
- Mouse Swiss Prot No:
- Q8C878
- Rat Gene Id:
- 117553
- Rat Swiss Prot No:
- Q99MI7
- Immunogen:
- Synthesized peptide derived from human UBA3 AA range: 105-155
- Specificity:
- This antibody detects endogenous levels of UBA3 at Human/Mouse/Rat
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500-2000
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Molecular Weight(Da):
- 51kD
- Background:
- The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
- Function:
- enzyme regulation:Binding of TP53BP2 to the regulatory subunit NAE1 decreases activity.,function:Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.,miscellaneous:Arg-211 acts as a selectivity gate, preventing misactivation of ubiquitin by this NEDD8-specific E1 complex.,pathway:Protein modification; protein neddylation.,similarity:Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.,subunit:Heterodimer of UBA3 and NAE1. Interacts with NEDD8, UBE2F and UBE2M. Binds ESR1 and ESR2 with bound steroid ligand.,tissue specificity:Ubiquitousl
- Subcellular Location:
- nucleus,cytosol,
- Expression:
- Ubiquitously expressed.
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- Antibody-FAQs
- Products Images
- Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°over night