• Target：
• PLCD3

• Fields：
• >>Inositol phosphate metabolism;>>Metabolic pathways;>>Calcium signaling pathway;>>Phosphatidylinositol signaling system;>>Thyroid hormone signaling pathway;>>AGE-RAGE signaling pathway in diabetic complications;>>Shigellosis

• Gene Name：
• PLCD3 KIAA1964

• Protein Name：
• PLCD3

• Human Gene Id：
• 113026

• Human Swiss Prot No：
• Q8N3E9

• Mouse Gene Id：
• 72469

• Mouse Swiss Prot No：
• Q8K2J0

• Immunogen：
• Synthesized peptide derived from human PLCD3 AA range: 501-551

• Specificity：
• This antibody detects endogenous levels of PLCD3 at Human/Mouse

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500-2000;IHC 1:50-300

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Molecular Weight(Da)：
• 87kD

• Background：
• This gene encodes a member of the phospholipase C family, which catalyze the hydrolysis of phosphatidylinositol 4,5-bisphosphate to generate the second messengers diacylglycerol and inositol 1,4,5-trisphosphate (IP3). Diacylglycerol and IP3 mediate a variety of cellular responses to extracellular stimuli by inducing protein kinase C and increasing cytosolic Ca(2+) concentrations. This enzyme localizes to the plasma membrane and requires calcium for activation. Its activity is inhibited by spermine, sphingosine, and several phospholipids. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.,cofactor:Binds 3 calcium ions per subunit. Two of the calcium ions are bound to the C2 domain.,domain:The C2 domain is a Ca(2+)-dependent membrane-targeting module.,domain:The PH domain mediates interaction with the surface membrane by binding to PIP2.,enzyme regulation:Strongly activated by phosphatidic acid. Inhibited by phosphatidylethanolamine (PtdEtn), phosphatidylcholine (PtdCho), sphingomyelin and phosphatidylserine (PtdSer).,function:Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca(2+) from intracellular stores. Essential for trophoblast and placental development.

• Subcellular Location：
• Membrane; Peripheral membrane protein. Cytoplasm. Cleavage furrow . Localizes at the cleavage furrow during cytokinesis. .

• Expression：
• Present in corneal epithelial cells (at protein level).

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