• Target：
• DEN1B

• Gene Name：
• DENND1B C1orf218 FAM31B

• Protein Name：
• DEN1B

• Human Gene Id：
• 163486

• Human Swiss Prot No：
• Q6P3S1

• Mouse Gene Id：
• 329260

• Mouse Swiss Prot No：
• Q3U1T9

• Immunogen：
• Synthesized peptide derived from human DEN1B AA range: 325-375

• Specificity：
• This antibody detects endogenous levels of DEN1B at Human/Mouse

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1：500-2000

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Molecular Weight(Da)：
• 47kD

• Background：
• Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM, Nov 2010],

• Function：
• PTM:Isoform 5 is phosphorylated on Tyr-475 and Tyr-484.,similarity:Contains 1 dDENN domain.,similarity:Contains 1 DENN domain.,similarity:Contains 1 uDENN domain.,

• Subcellular Location：
• Cytoplasm, cytosol . Cytoplasmic vesicle, clathrin-coated vesicle .

• Expression：
• Highly expressed in dendritic and natural killer cells and at lower levels in other myeloid lineage cells and in pituitary. Significantly up-regulated in effector memory T-cells as compared with naive T-cells.

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