PIGZ rabbit pAb
- Catalog No.:YT6506
- Applications:WB;ELISA;IHC
- Reactivity:Human;Mouse
- Target:
- PIGZ
- Fields:
- >>Glycosylphosphatidylinositol (GPI)-anchor biosynthesis
- Gene Name:
- PIGZ SMP3
- Protein Name:
- PIGZ
- Human Gene Id:
- 80235
- Human Swiss Prot No:
- Q86VD9
- Mouse Gene Id:
- 239827
- Mouse Swiss Prot No:
- Q8BTP0
- Immunogen:
- Synthesized peptide derived from human PIGZ AA range: 251-301
- Specificity:
- This antibody detects endogenous levels of PIGZ at Human/Mouse
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Molecular Weight(Da):
- 64kD
- Background:
- The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells that serves to anchor proteins to the cell surface. This gene encodes a protein that is localized to the endoplasmic reticulum, and is involved in GPI anchor biosynthesis. As shown for the yeast homolog, which is a member of a family of dolichol-phosphate-mannose (Dol-P-Man)-dependent mannosyltransferases, this protein can also add a side-branching fourth mannose to GPI precursors during the assembly of GPI anchors. [provided by RefSeq, Jul 2008],
- Function:
- caution:It is uncertain whether Met-1 or Met-32 is the initiator.,function:Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers a fourth mannose to some trimannosyl-GPIs during GPI precursor assembly. The presence of a fourth mannose in GPI is facultative and only scarcely detected, suggesting that it only exists in some tissues.,pathway:Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.,similarity:Belongs to the glycosyltransferase 22 family. PIGZ subfamily.,tissue specificity:Widely expressed at low level, with highest level in brain and colon.,
- Subcellular Location:
- Endoplasmic reticulum membrane ; Multi-pass membrane protein .
- Expression:
- Widely expressed at low level, with highest level in brain and colon.
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- Antibody-FAQs
- Products Images
- Western blot analysis of lysates from THP-1 cells, primary antibody was diluted at 1:1000, 4°over night
- Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).