Flotillin-1 Polyclonal Antibody
- Catalog No.:YT6292
- Applications:WB;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- Flotillin-1
- Fields:
- >>Insulin signaling pathway
- Gene Name:
- FLOT1
- Protein Name:
- Flotillin-1
- Human Gene Id:
- 10211
- Human Swiss Prot No:
- O75955
- Immunogen:
- Synthesized peptide derived from human Flotillin-1
- Specificity:
- This antibody detects endogenous levels of human Flotillin-1
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500-2000, ELISA(peptide)1:5000-20000
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- Flotillin-1
- Observed Band(KD):
- 48kD
- Background:
- This gene encodes an protein that localizes to the caveolae, which are small domains on the inner cell membranes. This protein plays a role in vesicle trafficking and cell morphology. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016],
- Function:
- function:May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.,similarity:Belongs to the band 7/mec-2 family. Flotillin subfamily.,subcellular location:Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2.,
- Subcellular Location:
- Cell membrane; Peripheral membrane protein . Endosome . Membrane, caveola ; Peripheral membrane protein . Melanosome . Membrane raft . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Membrane-associated protein of caveola (By similarity). .
- Expression:
- Adipose tissue,Adrenal gland,Lung,Peripheral blood leukocyte,Umbilical cord blood,
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- Antibody-FAQs
- Products Images
- Western blot analysis of various lysates, primary antibody was diluted at 1:1000, 4° over night, secondary antibody(cat: RS23920)was diluted at 1:10000, 37° 1hour.