HMG-I/HMG-Y Polyclonal Antibody

    • Catalog No.:YT6280
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • HMG-I/HMG-Y
      • Gene Name:
      • HMGA1 HMGIY
      • Protein Name:
      • HMG-I/HMG-Y
      • Human Gene Id:
      • 3159
      • Human Swiss Prot No:
      • P17096
      • Immunogen:
      • Synthesized peptide derived from human HMG-I/HMG-Y . AA20-60
      • Specificity:
      • This antibody detects endogenous levels of human HMG-I/HMG-Y
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • High mobility group protein HMG-I/HMG-Y (HMG-I(Y);High mobility group AT-hook protein 1;High mobility group protein A1;High mobility group protein R)
      • Molecular Weight(Da):
      • 12kD
      • Background:
      • This gene encodes a chromatin-associated protein involved in the regulation of gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of AT-rich regions in double-stranded DNA. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been identified on multiple chromosomes. [provided by RefSeq, Jan 2016],
      • Function:
      • disease:A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51L1.,function:HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.,mass spectrometry:With 1 acetyl and 2 phosphate groups PubMed:15302935,mass spectrometry:With 1 acetyl and 3 phosphate groups PubMed:15302935,mass spectrometry:With 1 acetyl, 1 methyl and 2 phosphate groups PubMed:15302935,mass spectrometry:With 1 acetyl, 1 methyl and 3 phosphate groups PubMed:15302935,mass spectrometry:With 1 acetyl, 2 methyl and 2 phosphate groups PubMed:15302935,mass spectrometry:With 1 acetyl,
      • Subcellular Location:
      • Nucleus. Chromosome.
      • Expression:
      • B-cell,Colon,Epithelium,Kidney,Liver,Mammary gland,Muscle,Pancreas,Placenta
      • Products Images
      • Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°over night
      • Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).