ITK Polyclonal Antibody
- Catalog No.:YT6225
- Applications:IHC;IF;WB
- Reactivity:Human;Mouse
- Target:
- ITK
- Fields:
- >>Chemokine signaling pathway;>>T cell receptor signaling pathway;>>Leukocyte transendothelial migration
- Gene Name:
- ITK EMT LYK
- Protein Name:
- ITK
- Human Gene Id:
- 3702
- Human Swiss Prot No:
- Q08881
- Immunogen:
- Synthesized peptide derived from human ITK AA range: 450-530
- Specificity:
- This antibody detects endogenous levels of human ITK
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- IHC 1:50-200, WB 1:500-2000. IF 1:50-200
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- Tyrosine-protein kinase ITK/TSK (EC 2.7.10.2;Interleukin-2-inducible T-cell kinase;IL-2-inducible T-cell kinase;Kinase EMT;T-cell-specific kinase;Tyrosine-protein kinase Lyk)
- Observed Band(KD):
- 72kD
- Background:
- This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq, Jul 2008],
- Function:
- catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,cofactor:Binds 1 zinc ion per subunit.,function:Plays a role in T-cell proliferation and differentiation.,induction:By interleukin-2.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.,similarity:Contains 1 Btk-type zinc finger.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,subcellular location:Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T-cells.,tissue specificity:T-cell lines and natural killer cell lines.,
- Subcellular Location:
- Cytoplasm . Nucleus . Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimulation.
- Expression:
- T-cell lines and natural killer cell lines.
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- Antibody-FAQs
- Products Images
- Immunohistochemical analysis of paraffin-embedded human brain tumor. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).