AATC Polyclonal Antibody

    • Catalog No.:YT6181
    • Applications:IHC;IF;WB
    • Reactivity:Human;Mouse;Rat
      • Target:
      • AATC
      • Fields:
      • >>Arginine biosynthesis;>>Alanine, aspartate and glutamate metabolism;>>Cysteine and methionine metabolism;>>Arginine and proline metabolism;>>Tyrosine metabolism;>>Phenylalanine metabolism;>>Phenylalanine, tyrosine and tryptophan biosynthesis;>>Metabolic pathways;>>Carbon metabolism;>>2-Oxocarboxylic acid metabolism;>>Biosynthesis of amino acids
      • Gene Name:
      • GOT1
      • Protein Name:
      • AATC
      • Human Gene Id:
      • 2805
      • Human Swiss Prot No:
      • P17174
      • Immunogen:
      • Synthesized peptide derived from human AATC
      • Specificity:
      • This antibody detects endogenous levels of human AATC
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • IHC 1:50-200, WB 1:500-2000. IF 1:50-200
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • Aspartate aminotransferase, cytoplasmic (EC 2.6.1.1;Glutamate oxaloacetate transaminase 1;Transaminase A)
      • Observed Band(KD):
      • 42kD
      • Background:
      • Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008],
      • Function:
      • catalytic activity:L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.,cofactor:Pyridoxal phosphate.,miscellaneous:In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.,similarity:Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.,subunit:Homodimer.,
      • Subcellular Location:
      • Cytoplasm .
      • Expression:
      • Brain,Liver,Lung,
      • Products Images
      • Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°over night
      • Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).