IDHP Polyclonal Antibody
- Catalog No.:YT6168
- Applications:IHC;IF;WB
- Reactivity:Human;Mouse;Rat
- Target:
- IDHP
- Fields:
- >>Citrate cycle (TCA cycle);>>Glutathione metabolism;>>Metabolic pathways;>>Carbon metabolism;>>2-Oxocarboxylic acid metabolism;>>Biosynthesis of amino acids;>>Peroxisome;>>Central carbon metabolism in cancer
- Gene Name:
- IDH2
- Protein Name:
- IDHP
- Human Gene Id:
- 3418
- Human Swiss Prot No:
- P48735
- Immunogen:
- Synthesized peptide derived from human IDHP
- Specificity:
- This antibody detects endogenous levels of human IDHP
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- IHC 1:50-200, WB 1:500-2000. IF 1:50-200
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- Isocitrate dehydrogenase [NADP], mitochondrial (IDH;EC 1.1.1.42;ICD-M;IDP;NADP(+)-specific ICDH;Oxalosuccinate decarboxylase)
- Observed Band(KD):
- 48kD
- Background:
- Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants. [provided by Ref
- Function:
- catalytic activity:Isocitrate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH.,catalytic activity:Oxalosuccinate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH.,cofactor:Binds 1 magnesium or manganese ion per subunit.,function:Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.,similarity:Belongs to the isocitrate and isopropylmalate dehydrogenases family.,subunit:Homodimer.,
- Subcellular Location:
- Mitochondrion .
- Expression:
- Colon,Heart,Kidney,Skin,
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- May 20-2022
- Cell-Based ELISA│解您多样本WB检测之困扰
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).