CIS Polyclonal Antibody
- Catalog No.:YT5920
- Applications:IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- CIS
- Fields:
- >>JAK-STAT signaling pathway;>>Prolactin signaling pathway
- Gene Name:
- CISH G18
- Protein Name:
- Cytokine-inducible SH2-containing protein (CIS) (CIS-1) (Protein G18) (Suppressor of cytokine signaling) (SOCS)
- Human Gene Id:
- 1154
- Human Swiss Prot No:
- Q9NSE2
- Mouse Gene Id:
- 12700
- Mouse Swiss Prot No:
- Q62225
- Rat Swiss Prot No:
- O70512
- Immunogen:
- Synthetic peptide from human protein at AA range: 30-90
- Specificity:
- The antibody detects endogenous CIS
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- IHC 1:50-200, ELISA 1:10000-20000. IF 1:50-200
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- Cytokine-inducible SH2-containing protein (CIS;CIS-1;Protein G18;Suppressor of cytokine signaling;SOCS)
- Background:
- cytokine inducible SH2 containing protein(CISH) Homo sapiens The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008],
- Function:
- disease:CISH deletion may be involved in the pathogenesis of renal cell carcinomas and of lung cancer since lung and kidney tumors frequently exhibit 3p21.3 deletions.,function:SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.,induction:By a subset of cytokines including erythropoietin (EPO).,pathway:Protein modification; protein ubiquitination.,PTM:Association wi
- Subcellular Location:
- cytoplasm,cytosol,plasma membrane,
- Expression:
- Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct.
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- Antibody-FAQs
- Products Images
- Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:200