• Target：
• SOCS-3

• Fields：
• >>Ubiquitin mediated proteolysis;>>Osteoclast differentiation;>>JAK-STAT signaling pathway;>>TNF signaling pathway;>>Insulin signaling pathway;>>Prolactin signaling pathway;>>Adipocytokine signaling pathway;>>Type II diabetes mellitus;>>Insulin resistance;>>Non-alcoholic fatty liver disease;>>Growth hormone synthesis, secretion and action;>>Hepatitis C;>>Influenza A;>>Herpes simplex virus 1 infection

• Gene Name：
• SOCS3 CIS3 SSI3

• Protein Name：
• Suppressor of cytokine signaling 3 (SOCS-3) (Cytokine-inducible SH2 protein 3) (CIS-3) (STAT-induced STAT inhibitor 3) (SSI-3)

• Human Gene Id：
• 9021

• Human Swiss Prot No：
• O14543

• Mouse Gene Id：
• 12702

• Mouse Swiss Prot No：
• O35718

• Rat Swiss Prot No：
• O88583

• Immunogen：
• Synthetic peptide from human protein at AA range: 20-70

• Specificity：
• The antibody detects endogenous SOCS-3

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000.IHC:1:50-300.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• Suppressor of cytokine signaling 3 (SOCS-3;Cytokine-inducible SH2 protein 3;CIS-3;STAT-induced STAT inhibitor 3;SSI-3)

• Molecular Weight(Da)：
• 25kD

• Background：
• This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development. [provided by RefSeq, Jul 2008],

• Function：
• disease:Genetic variation in the promoter region of SOCS3 may be associated with susceptibility to atopic dermatitis 4 (ATOD4) [MIM:605805]. Atopic dermatitis [MIM:603165], also known as eczema commonly begins in infancy or early childhook and is characterized by ichy and inflamed skin.,domain:The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.,domain:The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.,function:SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors includ

• Subcellular Location：
• intracellular,cytoplasm,cytosol,

• Expression：
• Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.

Lack of SOCS3 increases LPS-induced murine acute lung injury through modulation of Ly6C(+) macrophages. RESPIRATORY RESEARCH Resp Res. 2017 Dec;18(1):1-14 WB Mouse 1:500 bone marrow-derived macrophages (BMDMs)

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