COL25A1 Polyclonal Antibody

    • Catalog No.:YT5824
    • Applications:WB;ELISA
    • Reactivity:Human;Mouse
      • Target:
      • COL25A1
      • Fields:
      • >>Protein digestion and absorption
      • Gene Name:
      • COL25A1
      • Protein Name:
      • collagen, type XXV, alpha 1
      • Human Swiss Prot No:
      • Q9BXS0
      • Mouse Swiss Prot No:
      • Q99MQ5
      • Immunogen:
      • Synthetic peptide from human protein at AA range: 101-150
      • Specificity:
      • The antibody detects endogenous COL25A1 protein
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500-2000, ELISA 1:10000-20000
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • COL25A1
      • Observed Band(KD):
      • 64kD
      • Background:
      • This gene encodes a brain-specific membrane associated collagen. A product of proteolytic processing of the encoded protein, CLAC (collagenous Alzheimer amyloid plaque component), binds to amyloid beta-peptides found in Alzheimer amyloid plaques but CLAC inhibits rather than facilitates amyloid fibril elongation (PMID: 16300410). A study of over-expression of this collagen in mice, however, found changes in pathology and behavior suggesting that the encoded protein may promote amyloid plaque formation (PMID: 19548013). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011],
      • Function:
      • caution:The pyrrolidone carboxylic acid reported in PubMed:11927537 probably formed artifactually from Glu-113 during the extraction procedure in 70% formic acid. In PubMed:15522881, the protein was found to have unblocked Glu at the N-terminus.,function:Inhibits fibrillization of beta amyloid peptide during the elongation phase. Has also been shown to assemble amyloid fibrils into protease-resistant aggregates. Binds heparin.,PTM:Glycosylated.,PTM:Hydroxylated on 11% of proline residues and 49% of lysine residues.,PTM:Undergoes proteolytic cleavage by furin protease to yield the soluble collagen-like Alzheimer amyloid plaque component.,similarity:Contains 7 collagen-like domains.,subcellular location:After proteolytic cleavage, CLAC is secreted.,subunit:Forms homodimers and homotrimers. Binds to the fibrillized forms of beta amyloid peptide 40 (beta-APP40) and beta amyloid peptide 42 (b
      • Subcellular Location:
      • Membrane ; Single-pass type II membrane protein . After proteolytic cleavage, CLAC is secreted.
      • Expression:
      • Expressed predominantly in brain. Deposited preferentially in primitive or neuritic amyloid plaques which are typical of Alzheimer disease.
      • Products Images
      • Western Blot analysis of HEPG2 cells using Antibody diluted at 500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000