NHERF-2 Polyclonal Antibody

    • Catalog No.:YT5676
    • Applications:WB;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • NHERF-2
      • Fields:
      • >>Aldosterone-regulated sodium reabsorption
      • Gene Name:
      • SLC9A3R2
      • Protein Name:
      • Na(+)/H(+) exchange regulatory cofactor NHE-RF2
      • Human Gene Id:
      • 9351
      • Human Swiss Prot No:
      • Q15599
      • Mouse Swiss Prot No:
      • Q9JHL1
      • Immunogen:
      • Synthesized peptide derived from NHERF-2 . at AA range: 20-100
      • Specificity:
      • NHERF-2 Polyclonal Antibody detects endogenous levels of NHERF-2 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • SLC9A3R2;NHERF2;Na(+)/H(+) exchange regulatory cofactor NHE-RF2;NHERF-2;NHE3 kinase A regulatory protein E3KARP;SRY-interacting protein 1;SIP-1;Sodium-hydrogen exchanger regulatory factor 2;Solute carrier family 9 isoform A3 regulatory factor 2;Tyrosine kinase activator protein 1;TKA-1
      • Observed Band(KD):
      • 37kD
      • Background:
      • This gene encodes a member of the NHERF family of PDZ scaffolding proteins. These proteins mediate many cellular processes by binding to and regulating the membrane expression and protein-protein interactions of membrane receptors and transport proteins. The encoded protein plays a role in intestinal sodium absorption by regulating the activity of the sodium/hydrogen exchanger 3, and may also regulate the cystic fibrosis transmembrane regulator (CFTR) ion channel. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011],
      • Function:
      • function:Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus.,similarity:Contains 2 PDZ (DHR) domains.,subcellular location:Nuclear, in a punctate pattern.,subunit:Homodimer, and heterodimer with SLC9A3R1. Binds PODXL and PDZK1 (By similarity). Binds ADRB2, SLC9A3, P2RY1, P2YR2, SRY, RDX and LPAR2.,tissue specificity:Widely expressed.,
      • Subcellular Location:
      • Endomembrane system ; Peripheral membrane protein . Nucleus . Apical cell membrane . Localizes with EZR and PODXL at the apical cell membrane of glomerular epithelium cells and the sides of the food processes (By similarity). Nuclear, in a punctate pattern. .
      • Expression:
      • Widely expressed.
      • Products Images
      • Western blot analysis of JK K562 using NHERF-2 antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000