MRP-L35 Polyclonal Antibody

    • Catalog No.:YT5669
    • Applications:WB;IHC
    • Reactivity:Human;Mouse
      • Target:
      • MRP-L35
      • Fields:
      • >>Ribosome
      • Gene Name:
      • MRPL35
      • Protein Name:
      • 39S ribosomal protein L35 mitochondrial
      • Human Swiss Prot No:
      • Q9NZE8
      • Mouse Swiss Prot No:
      • Q9CQL6
      • Immunogen:
      • Synthesized peptide derived from MRP-L35 . at AA range: 80-160
      • Specificity:
      • MRP-L35 Polyclonal Antibody detects endogenous levels of MRP-L35 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500-2000;IHC 1:50-300
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • MRPL35;BM-007;39S ribosomal protein L35, mitochondrial;L35mt;MRP-L35
      • Observed Band(KD):
      • 21kD
      • Background:
      • Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 6p, 10q, and Xp. [provided by RefSeq, Jul 2008],
      • Function:
      • similarity:Belongs to the ribosomal protein L35P family.,
      • Subcellular Location:
      • Mitochondrion .
      • Expression:
      • Bone marrow,Liver,Stomach,
      • Products Images
      • Western blot analysis of 293T COLO using MRP-L35 antibody. Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
      • Western blot analysis of 293 using MRP-L35 antibody. Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
      • Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).