MRP-L35 Polyclonal Antibody
- Catalog No.:YT5669
- Applications:WB;IHC
- Reactivity:Human;Mouse
- Target:
- MRP-L35
- Fields:
- >>Ribosome
- Gene Name:
- MRPL35
- Protein Name:
- 39S ribosomal protein L35 mitochondrial
- Human Gene Id:
- 51318
- Human Swiss Prot No:
- Q9NZE8
- Mouse Gene Id:
- 66223
- Mouse Swiss Prot No:
- Q9CQL6
- Immunogen:
- Synthesized peptide derived from MRP-L35 . at AA range: 80-160
- Specificity:
- MRP-L35 Polyclonal Antibody detects endogenous levels of MRP-L35 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500-2000;IHC 1:50-300
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- MRPL35;BM-007;39S ribosomal protein L35, mitochondrial;L35mt;MRP-L35
- Observed Band(KD):
- 21kD
- Background:
- Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 6p, 10q, and Xp. [provided by RefSeq, Jul 2008],
- Function:
- similarity:Belongs to the ribosomal protein L35P family.,
- Subcellular Location:
- Mitochondrion .
- Expression:
- Bone marrow,Liver,Stomach,
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- Antibody-FAQs
- Products Images
- Western blot analysis of 293T COLO using MRP-L35 antibody. Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- Western blot analysis of 293 using MRP-L35 antibody. Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).