Glut4 Polyclonal Antibody

    • Catalog No.:YT5523
    • Applications:WB,IHC-p,ELISA
    • Reactivity:Human,Mouse,Rat
    • Description
    • Gene Name:
    • SLC2A4
    • Protein Name:
    • Solute carrier family 2 facilitated glucose transporter member 4
    • Human Gene Id:
    • 6517
    • Human Swiss Prot No:
    • P14672
    • Mouse Swiss Prot No:
    • P14142
    • Immunogen:
    • The antiserum was produced against synthesized peptide derived from the N-terminal region of human SLC2A4. AA range:21-70
    • Specificity:
    • Glut4 Polyclonal Antibody detects endogenous levels of Glut4 protein.
    • Formulation:
    • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    • Source:
    • Rabbit
    • Dilution:
    • WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300
    • Purification:
    • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    • Concentration:
    • 1 mg/ml
    • Storage Stability:
    • -20°C/1 year
    • Other Name:
    • SLC2A4; GLUT4; Solute carrier family 2, facilitated glucose transporter member 4; Glucose transporter type 4, insulin-responsive; GLUT-4
    • MolecularWeight(Da):
    • 54787
    • Observed Band(KD):
    • 56
    • Background:
    • SLC2A4 is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM).
    • Function:
    • carbohydrate transport, hexose transport, monosaccharide transport, glucose transport, carbohydrate homeostasis,homeostatic process, glucose homeostasis, fat cell differentiation, glucose import, chemical homeostasis, brown fat cell differentiation, transmembrane transport,
    • Products Images
    • Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,Glut4 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    • Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,Glut4 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    • Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,Glut4 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    • Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,Glut4 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    • Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,Glut4 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    • Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,Glut4 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    • Western Blot analysis of SKOV3 cells using Glut4 Polyclonal Antibody.. Secondary antibody(catalog#:RS0002) was diluted at 1:20000