Glut4 Polyclonal Antibody

    • Catalog No.:YT5523
    • Applications:WB,IHC-p,ELISA
    • Reactivity:Human,Mouse,Rat
      • Gene Name:
      • SLC2A4
      • Protein Name:
      • Solute carrier family 2 facilitated glucose transporter member 4
      • Human Gene Id:
      • 6517
      • Human Swiss Prot No:
      • P14672
      • Mouse Swiss Prot No:
      • P14142
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from the N-terminal region of human SLC2A4. AA range:21-70
      • Specificity:
      • Glut4 Polyclonal Antibody detects endogenous levels of Glut4 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • SLC2A4; GLUT4; Solute carrier family 2, facilitated glucose transporter member 4; Glucose transporter type 4, insulin-responsive; GLUT-4
      • MolecularWeight(Da):
      • 54787
      • Observed Band(KD):
      • 56
      • Background:
      • solute carrier family 2 member 4(SLC2A4) Homo sapiens This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM). [provided by RefSeq, Jul 2008],
      • Function:
      • disease:Defects in SLC2A4 may be a cause of noninsulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. Defects in SLC2A4 may be a cause of certain post-receptor defects in NIDDM. The variant in position Ile-383 is found in a small number of NIDDM patients, but seems not to be found in nondiabetic subjects.,function:Insulin-regulated facilitative glucose transporter.,miscellaneous:Insulin-stimulated phosphorylation of TBC1D4 is required for GLUT4 translocation.,online information:GLUT4 entry,PTM:Sumoylated.,similarity:Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.,subcellular location:Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration.,subunit:Binds to DAX
      • Subcellular Location:
      • multivesicular body,cytosol,plasma membrane,integral component of plasma membrane,clathrin-coated pit,external side of plasma membrane,endomembrane system,vesicle membrane,membrane,integral component of membrane,clathrin-co
      • Expression:
      • Colon,

      Zhang, Xueyan, et al. "Targeting glycometabolic reprogramming to restore the sensitivity of leukemia drug-resistant K562/ADM cells to adriamycin." Life sciences 215 (2018): 1-10.
      Catalog No.:YT5523

      Zhang, Xueyan, et al. "Glycometabolic adaptation mediates the insensitivity of drug-resistant K562/ADM leukaemia cells to adriamycin via the AKT-mTOR/c-Myc signalling pathway." Molecular medicine reports 15.4 (2017): 1869-1876.
      Catalog No.:YT5523

      • Products Images
      • Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,Glut4 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,Glut4 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,Glut4 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,Glut4 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,Glut4 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,Glut4 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Western Blot analysis of SKOV3 cells using Glut4 Polyclonal Antibody.. Secondary antibody(catalog#:RS0002) was diluted at 1:20000