• Target：
• Cryopyrin

• Fields：
• >>Necroptosis;>>NOD-like receptor signaling pathway;>>C-type lectin receptor signaling pathway;>>Pathogenic Escherichia coli infection;>>Shigellosis;>>Salmonella infection;>>Pertussis;>>Yersinia infection;>>Influenza A;>>Coronavirus disease - COVID-19;>>Lipid and atherosclerosis

• Gene Name：
• NLRP3

• Protein Name：
• NACHT LRR and PYD domains-containing protein 3

• Human Gene Id：
• 114548

• Human Swiss Prot No：
• Q96P20

• Mouse Swiss Prot No：
• Q8R4B8

• Immunogen：
• The antiserum was produced against synthesized peptide derived from the Internal region of human NLRP3. AA range:511-560

• Specificity：
• Cryopyrin Polyclonal Antibody detects endogenous levels of Cryopyrin protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• NLRP3;C1orf7;CIAS1;NALP3;PYPAF1;NACHT, LRR and PYD domains-containing protein 3;Angiotensin/vasopressin receptor AII/AVP-like;Caterpiller protein 1.1CLR1.1;Cold autoinflammatory syndrome 1 protein;Cryopyrin;PYRIN-containing APAF1-like protein 1

• Observed Band(KD)：
• 115kD

• Background：
• This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are s

• Function：
• disease:Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) [MIM:191900]; also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs.,disease:Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) [MIM:607115]; also known as 'neonatal onset multisystem inflammatory disease,' or NOMID, a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis, and joint manifestations with recurrent fever and inflammation.,disease:Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) [MIM:120100]; commonly known as fami

• Subcellular Location：
• Cytoplasm, cytosol . Inflammasome . Endoplasmic reticulum . Secreted . Nucleus . In macrophages, under resting conditions, mainly located in the cytosol, on the endoplasmic reticulum. After stimulation with inducers of the NLRP3 inflammasome, mitochondria redistribute in the vicinity of the endoplasmic reticulum in the perinuclear region, which results in colocalization of NLRP3 on the endoplasmic reticulum and PYCARD on mitochondria, allowing the activation of inflammasome assembly. After the induction of pyroptosis, inflammasome specks are released into the extracellular space where they can further promote IL1B processing and where they can be engulfed by macrophages. Phagocytosis induces lysosomal damage and inflammasome activation in the recipient cells (PubMed:24952504). In the Th2 s

• Expression：
• Predominantly expressed in macrophages (PubMed:33231615, PubMed:34133077). Also expressed in dendritic cells, B- and T-cells (at protein level) (PubMed:11786556) (PubMed:17164409). Expressed in LPS-treated granulocytes, but not in resting cells (at protein level) (PubMed:17164409). Expression in monocytes is very weak (at protein level) (PubMed:17164409). Expressed in stratified non-keratinizing squamous epithelium, including oral, esophageal and ectocervical mucosa and in the Hassall's corpuscles in the thymus. Also, detected in the stratified epithelium covering the bladder and ureter (transitional mucosa) (at protein level) (PubMed:17164409). Expressed in lung epithelial cells (at protein level) (PubMed:23229815). Expressed in chondrocytes (PubMed:12032915). Expressed at low levels in r

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Gynostemma pentaphyllum polysaccharides ameliorate non-alcoholic steatohepatitis in mice associated with gut microbiota and the TLR2/NLRP3 pathway Frontiers in Endocrinology Rui-Rui Wang IHC Mouse

Gut-Flora-Dependent Metabolite Trimethylamine-N-Oxide Promotes Atherosclerosis-Associated Inflammation Responses by Indirect ROS Stimulation and Signaling Involving AMPK and SIRT1 Nutrients Wenjian Ma WB Human

IL-37 alleviates TNF-α-induced pyroptosis of rheumatoid arthritis fibroblast-like synoviocytes by inhibiting the NF-κB/GSDMD signaling pathway IMMUNOBIOLOGY Qiang Shu IHC Human synovial tissue

Hypoxic preconditioned mesenchymal stem cells ameliorate rat brain injury after cardiopulmonary resuscitation by suppressing neuronal pyroptosis. Feng Chen WB Rat cortical tissue

miR-20b attenuates airway inflammation by regulating TXNIP and NLRP3 inflammasome in ovalbumin-induced asthmatic mice. Yun Sun IF Mouse 1:100 Lung tissue

Induced Neural Stem Cells Suppressed Neuroinflammation by Inhibiting the Microglial Pyroptotic Pathway in Intracerebral Hemorrhage Rats. Xueyun Liang IF Rat,Human 1:200 brain tissue induced neural stem cells (iNSCs)

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Pristimerin suppresses AIM2 inflammasome by modulating AIM2-PYCARD/ASC stability via selective autophagy to alleviate tendinopathy. Autophagy Xiao Yu WB Mouse Achilles tendon Peritoneal macrophages

A novel drug combination of Tofacitinib and Iguratimod alleviates rheumatoid arthritis and secondary osteoporosis. INTERNATIONAL IMMUNOPHARMACOLOGY Qiang Shu IHC，IF Rat 1:100，1:100 Synovial tissue,bone tissue

Yueliang Yin Ameliorates Endometrial Receptivity in Mice with Embryo Implantation Failure by Reducing Pyroptosis and Activating BDNF/TrkB Pathway. MOLECULAR NUTRITION & FOOD RESEARCH Ting Wang WB Mouse uterus

Piceatannol alleviates liver ischaemia/reperfusion injury by inhibiting TLR4/NF-κB/NLRP3 in hepatic macrophages. EUROPEAN JOURNAL OF PHARMACOLOGY Jian Du IHC，IF，WB Mouse 1:100,1:50,1:1000 liver tissue macrophages

Chlorogenic acid protects against myocardial ischemia–reperfusion injury in mice by inhibiting Lnc Neat1/NLRP3 inflammasome-mediated pyroptosis. Scientific Reports Deqin Lu IHC Mouse cardiomyocytes

Nobiletin Alleviated Epithelial–Mesenchymal Transition of Hepatocytes in Liver Fibrosis Based on Autophagy—Hippo/YAP Pathway MOLECULAR NUTRITION & FOOD RESEARCH Yuting Hao WB Mouse,Human liver tissue L02 cell

Dihydroxyacetone phosphate accumulation leads to podocyte pyroptosis in diabetic kidney disease JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Zongwei Zhang WB Mouse kidney tissue podocytes

NLRP6 deficiency suppresses colorectal cancer liver metastasis growth by modulating M-MDSC-induced immunosuppressive microenvironment BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE Liangzheng Chang IHC Human colorectal cancer (CRC) tissue,liver metastasis tissue

Cathepsin B aggravates atherosclerosis in ApoE-deficient mice by modulating vascular smooth muscle cell pyroptosis through NF-κB / NLRP3 signaling pathway PLoS One Hui Li IHC,WB Mouse 1:200 aorta tissue vascular smooth muscle cells (VSMCs)

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