• Target：
• NUMB

• Fields：
• >>Notch signaling pathway

• Gene Name：
• NUMB

• Protein Name：
• Protein numb homolog

• Human Gene Id：
• 8650

• Human Swiss Prot No：
• P49757

• Mouse Gene Id：
• 18222

• Mouse Swiss Prot No：
• Q9QZS3

• Rat Swiss Prot No：
• Q2LC84

• Immunogen：
• Synthesized peptide derived from the Internal region of human NUMB.

• Specificity：
• NUMB Polyclonal Antibody detects endogenous levels of NUMB protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500-2000; IF ICC 1:100-500;ELISA 1:5000-20000

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• NUMB;Protein numb homolog;h-Numb;Protein S171

• Observed Band(KD)：
• 70kD

• Background：
• The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016],

• Function：
• function:Implicated in the control of cell fate decisions during development.,PTM:Isoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity). Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.,sequence caution:Intron retention.,similarity:Contains 1 PID domain.,subunit:Interacts with EPS15, LNX and NOTCH1. May interact with DUOXA1. Interacts with RALBP1 in a complex also containing EPN1 and TFAP2A during interphase and mitosis.,

• Subcellular Location：
• Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Localizes to perinuclear endosomes in an AAK1-dependent manner. .

• Expression：
• B cells,Blood,Brain,Cervix carcinoma,Epithelium,Fetal brain,HeLa cells,Live

Chemotherapy-elicited exosomal miR-378a-3p and miR-378d promote breast cancer stemness and chemoresistance via the activation of EZH2/STAT3 signaling. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH J Exp Clin Canc Res. 2021 Dec;40(1):1-18 IHC Mouse 1 : 100 CAL51 cell-Xenograft

Hepatic stem cell Numb gene is a potential target of Huang Qi Decoction against cholestatic liver fibrosis. Scientific Reports Sci Rep-Uk. 2020 Oct;10(1):1-14 IF Rat 1:50 Liver

Hepatic stem cell Numb gene is a potential target of Huang Qi Decoction against cholestatic liver fibrosis. Sci Rep 10, 17486 (2020). 

BM-MSCs overexpressing the Numb enhance the therapeutic effect on cholestatic liver fibrosis by inhibiting the ductular reaction Stem Cell Research & Therapy Yong-ping Mu WB,IHC Rat liver WB-F344 cell，bone marrow mesenchymal stem cells (BM-MSCs)

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