• Target：
• IDH3A

• Fields：
• >>Citrate cycle (TCA cycle);>>Metabolic pathways;>>Carbon metabolism;>>2-Oxocarboxylic acid metabolism;>>Biosynthesis of amino acids

• Gene Name：
• IDH3A

• Protein Name：
• Isocitrate dehydrogenase [NAD] subunit alpha mitochondrial

• Human Gene Id：
• 3419

• Human Swiss Prot No：
• P50213

• Mouse Gene Id：
• 67834

• Mouse Swiss Prot No：
• Q9D6R2

• Rat Gene Id：
• 114096

• Rat Swiss Prot No：
• Q99NA5

• Immunogen：
• Synthesized peptide derived from the Internal region of human IDH3A.

• Specificity：
• IDH3A Polyclonal Antibody detects endogenous levels of IDH3A protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• IDH3A;Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial;Isocitric dehydrogenase subunit alpha;NAD(+)-specific ICDH subunit alpha

• Observed Band(KD)：
• 39kD

• Background：
• Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:Isocitrate + NAD(+) = 2-oxoglutarate + CO(2) + NADH.,cofactor:Binds 1 magnesium or manganese ion per subunit.,similarity:Belongs to the isocitrate and isopropylmalate dehydrogenases family.,subunit:Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1.,

• Subcellular Location：
• Mitochondrion.

• Expression：
• Brain,Brain cortex,Cajal-Retzius cell,Esophagus tumor,Fetal

Effect of IDH3a on glucose uptake in lung adenocarcinoma: A pilot study based on [18F]FDG. Cancer Medicine 2019 Jul 29 WB Human 1:500 A549 cell, H1299 cell

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