IDH3A Polyclonal Antibody

    • Catalog No.:YT5318
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • IDH3A
      • Fields:
      • >>Citrate cycle (TCA cycle);>>Metabolic pathways;>>Carbon metabolism;>>2-Oxocarboxylic acid metabolism;>>Biosynthesis of amino acids
      • Gene Name:
      • IDH3A
      • Protein Name:
      • Isocitrate dehydrogenase [NAD] subunit alpha mitochondrial
      • Human Gene Id:
      • 3419
      • Human Swiss Prot No:
      • P50213
      • Mouse Swiss Prot No:
      • Q9D6R2
      • Immunogen:
      • Synthesized peptide derived from the Internal region of human IDH3A.
      • Specificity:
      • IDH3A Polyclonal Antibody detects endogenous levels of IDH3A protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC-p: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • IDH3A;Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial;Isocitric dehydrogenase subunit alpha;NAD(+)-specific ICDH subunit alpha
      • Observed Band(KD):
      • 39kD
      • Background:
      • Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. [provided by RefSeq, Jul 2008],
      • Function:
      • catalytic activity:Isocitrate + NAD(+) = 2-oxoglutarate + CO(2) + NADH.,cofactor:Binds 1 magnesium or manganese ion per subunit.,similarity:Belongs to the isocitrate and isopropylmalate dehydrogenases family.,subunit:Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1.,
      • Subcellular Location:
      • Mitochondrion.
      • Expression:
      • Brain,Brain cortex,Cajal-Retzius cell,Esophagus tumor,Fetal
      • Products Images
      • Western Blot analysis of MCF-7, HT29, mouse brain, mouse heart, mouse colon, mouse kidney, mouse skeletal muscle cells using IDH3A Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
      • Immunohistochemical analysis of paraffin-embedded human-lymph-gland, antibody was diluted at 1:100